PMID- 16375902
OWN - NLM
STAT- MEDLINE
DCOM- 20060530
LR  - 20071114
IS  - 0016-6480 (Print)
IS  - 0016-6480 (Linking)
VI  - 146
IP  - 2
DP  - 2006 Apr
TI  - Differential expression of tuberoinfundibular peptide 38 and 
      glucose-6-phosphatase in tilapia.
PG  - 186-94
AB  - A new parathyroid hormone (PTH)-like endocrine system has been identified in 
      mammals and fishes consisting of the PTH type-2 receptor (PTH2R) and 
      tuberoinfundibular peptide 39 (TIP39). Although the mammalian PTH2R-TIP39 system 
      is involved in nociception and pituitary regulation, the function(s) of this 
      system in fishes is undetermined. Using degenerate primers based on conserved 
      zebrafish and fugu TIP39 nucleotide sequences, 3'-RACE reactions isolated the 
      coding region of a putative TIP38 cDNA in the Nile tilapia (Oreochromis 
      niloticus). Tilapia-specific primers were subsequently used in 5'-RACE reactions 
      to isolate the remaining portion of the transcript. The cDNA encoding O. 
      niloticus TIP (OnTIP38) was determined to yield a 38 amino acid secreted hormone. 
      A second tilapia TIP38 cDNA was isolated from the euryhaline Mozambique tilapia 
      (OmTIP38). Except for the 39th residue, both tilapia cDNA sequences showed 
      significant identity to human, bovine, murine, fugu, and zebrafish TIP39. To 
      determine the tissue-specific expression of OnTIP38 and OmTIP38, real-time 
      quantitative RT-PCR (rQRT-PCR) was performed on skin, gill, kidney, testis, 
      heart, and brain. In freshwater (FW)-acclimated Nile tilapia, OnTIP38 showed 
      highest levels of expression in kidney and lowest levels in skin and gill. In 
      Mozambique tilapia tissues, expression of OmTIP38 and G6Pase (glucose-6 
      phosphatase) were higher in salt water (SW)-acclimated fish than in FW-acclimated 
      fish. G6Pase expression, and not OmTIP38, showed significant differences among 
      various tissues in FW- and SW-acclimated fish. Results of the present study 
      clearly indicate that the TIP38/39-PTH2R system shows considerable conservation 
      in sequence identity and tissue-specific expression in mammals and fishes.
FAU - Shoemaker, Justin M
AU  - Shoemaker JM
AD  - Department of Biological Sciences, Illinois State University, Normal, IL 61790, 
      USA.
FAU - Riley, Larry G
AU  - Riley LG
FAU - Hirano, Tetsuya
AU  - Hirano T
FAU - Grau, E Gordon
AU  - Grau EG
FAU - Rubin, David A
AU  - Rubin DA
LA  - eng
GR  - DK60513/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20051220
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (DNA, Complementary)
RN  - 0 (Neuropeptides)
RN  - 0 (Peptides)
RN  - 0 (Receptor, Parathyroid Hormone, Type 2)
RN  - 0 (tuberoinfundibular peptide 38)
RN  - 0 (tuberoinfundibular peptide 39)
RN  - EC 3.1.3.9 (Glucose-6-Phosphatase)
SB  - IM
MH  - *Adaptation, Physiological
MH  - Amino Acid Sequence
MH  - Animals
MH  - DNA, Complementary
MH  - Fresh Water
MH  - Gene Expression Profiling
MH  - Glucose-6-Phosphatase/*biosynthesis
MH  - Molecular Sequence Data
MH  - Neuropeptides/biosynthesis/genetics
MH  - Peptides/*metabolism
MH  - Receptor, Parathyroid Hormone, Type 2
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Seawater
MH  - Sequence Homology
MH  - Tilapia/*genetics
MH  - Tissue Distribution
EDAT- 2005/12/27 09:00
MHDA- 2006/05/31 09:00
CRDT- 2005/12/27 09:00
PHST- 2005/05/25 00:00 [received]
PHST- 2005/09/02 00:00 [revised]
PHST- 2005/10/22 00:00 [accepted]
PHST- 2005/12/27 09:00 [pubmed]
PHST- 2006/05/31 09:00 [medline]
PHST- 2005/12/27 09:00 [entrez]
AID - S0016-6480(05)00330-8 [pii]
AID - 10.1016/j.ygcen.2005.10.009 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2006 Apr;146(2):186-94. doi: 10.1016/j.ygcen.2005.10.009. 
      Epub 2005 Dec 20.