PMID- 16377170
OWN - NLM
STAT- MEDLINE
DCOM- 20060413
LR  - 20061115
IS  - 0958-1669 (Print)
IS  - 0958-1669 (Linking)
VI  - 17
IP  - 1
DP  - 2006 Feb
TI  - Linking microbial community structure with function: fluorescence in situ 
      hybridization-microautoradiography and isotope arrays.
PG  - 83-91
AB  - The ecophysiology of microorganisms has been at the heart of microbial ecology 
      since its early days, but only during the past decade have methods become 
      available for cultivation-independent, direct identification of microorganisms in 
      complex communities and for the simultaneous investigation of their activity and 
      substrate uptake patterns. The combination of fluorescence in situ hybridization 
      (FISH) and microautoradiography (MAR) is currently the most widely applied tool 
      for revealing physiological properties of microorganisms in their natural 
      environment with single-cell resolution. For example, this technique has been 
      used in wastewater treatment and marine systems to describe the functional 
      properties of newly discovered species, and to identify microorganisms 
      responsible for key physiological processes. Recently, the scope of FISH-MAR was 
      extended by rendering it quantitative and by combining it with microelectrode 
      measurements or stable isotope probing. Isotope arrays have also been developed 
      that exploit the parallel detection offered by DNA microarrays to measure 
      incorporation of labelled substrate into the rRNA of many community members in a 
      single experiment.
FAU - Wagner, Michael
AU  - Wagner M
AD  - Department of Microbial Ecology, University of Vienna, Althanstrasse 14, 1090 
      Wien, Austria. wagner@microbial-ecology.net
FAU - Nielsen, Per H
AU  - Nielsen PH
FAU - Loy, Alexander
AU  - Loy A
FAU - Nielsen, Jeppe L
AU  - Nielsen JL
FAU - Daims, Holger
AU  - Daims H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20051227
PL  - England
TA  - Curr Opin Biotechnol
JT  - Current opinion in biotechnology
JID - 9100492
RN  - 0 (DNA, Bacterial)
RN  - 0 (Isotopes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (Radioisotopes)
SB  - IM
MH  - Archaea/genetics/metabolism
MH  - Autoradiography
MH  - Bacteria/genetics/metabolism
MH  - DNA, Bacterial/analysis
MH  - *Environmental Microbiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Isotopes
MH  - Oligonucleotide Array Sequence Analysis
MH  - Plankton/genetics/metabolism
MH  - RNA, Bacterial/analysis
MH  - RNA, Ribosomal/analysis
MH  - Radioisotopes
RF  - 60
EDAT- 2005/12/27 09:00
MHDA- 2006/04/14 09:00
CRDT- 2005/12/27 09:00
PHST- 2005/10/04 00:00 [received]
PHST- 2005/11/15 00:00 [revised]
PHST- 2005/12/12 00:00 [accepted]
PHST- 2005/12/27 09:00 [pubmed]
PHST- 2006/04/14 09:00 [medline]
PHST- 2005/12/27 09:00 [entrez]
AID - S0958-1669(05)00198-9 [pii]
AID - 10.1016/j.copbio.2005.12.006 [doi]
PST - ppublish
SO  - Curr Opin Biotechnol. 2006 Feb;17(1):83-91. doi: 10.1016/j.copbio.2005.12.006. 
      Epub 2005 Dec 27.