PMID- 16378972
OWN - NLM
STAT- MEDLINE
DCOM- 20060127
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 80
IP  - 2
DP  - 2006 Jan
TI  - Defining the cellular target(s) of porcine reproductive and respiratory syndrome 
      virus blocking monoclonal antibody 7G10.
PG  - 689-96
AB  - We produced a monoclonal antibody (MAb) (7G10) that has blocking activity against 
      porcine reproductive and respiratory syndrome virus (PRRSV). In this study, we 
      identified the components of the 7G10 MAb-bound complex as cytoskeletal 
      filaments: vimentin, cytokeratin 8, cytokeratin 18, actin, and hair type II basic 
      keratin. Vimentin bound to PRRSV nucleocapsid protein and anti-vimentin 
      antibodies showed PRRSV-blocking activity. Vimentin was expressed on the surface 
      of MARC-145, a PRRSV-susceptible cell line. Simian vimentin rendered BHK-21 and 
      CRFK, nonsusceptible cell lines, susceptible to PRRSV infection. These results 
      suggest that vimentin is part of the PRRSV receptor complex and that it plays an 
      important role in PRRSV binding with the other cytoskeletal filaments that 
      mediate transportation of the virus in the cytosol.
FAU - Kim, Jeong-Ki
AU  - Kim JK
AD  - Louise C. Averill Research Laboratory, Department of Diagnostic 
      Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, 
      Manhattan, KS 66506, USA.
FAU - Fahad, Al-Majhdi
AU  - Fahad AM
FAU - Shanmukhappa, Kumar
AU  - Shanmukhappa K
FAU - Kapil, Sanjay
AU  - Kapil S
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Actins)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Vimentin)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Actins/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antibodies, Monoclonal/immunology
MH  - *Antibodies, Viral/immunology
MH  - Cell Line
MH  - Keratins/metabolism
MH  - Membrane Proteins/metabolism
MH  - Molecular Sequence Data
MH  - Nucleocapsid Proteins/metabolism
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*metabolism
MH  - Protein Binding
MH  - Sequence Alignment
MH  - Vimentin/genetics/*metabolism
PMC - PMC1346842
EDAT- 2005/12/28 09:00
MHDA- 2006/01/28 09:00
PMCR- 2006/05/01
CRDT- 2005/12/28 09:00
PHST- 2005/12/28 09:00 [pubmed]
PHST- 2006/01/28 09:00 [medline]
PHST- 2005/12/28 09:00 [entrez]
PHST- 2006/05/01 00:00 [pmc-release]
AID - 80/2/689 [pii]
AID - 1230-05 [pii]
AID - 10.1128/JVI.80.2.689-696.2006 [doi]
PST - ppublish
SO  - J Virol. 2006 Jan;80(2):689-96. doi: 10.1128/JVI.80.2.689-696.2006.