PMID- 16379541
OWN - NLM
STAT- MEDLINE
DCOM- 20060412
LR  - 20151119
IS  - 1090-6576 (Print)
IS  - 1090-6576 (Linking)
VI  - 9
IP  - 4
DP  - 2005 Winter
TI  - Development of an oligochip for genotyping human leukocyte antigen-B51 and its 
      clinical application.
PG  - 292-6
AB  - Human leukocyte antigen (HLA) has been associated with Behcet's disease (BD), 
      among which HLA-B51 is the most strongly associated genetic marker. The sandwich 
      hybridization technique was applied in the design of the specific oligonucleotide 
      probes to ensure the specific and accurate results. The probe-spotted chip was 
      hybridized with the polymerase chain reaction (PCR) amplicons including nine 
      suballeles (B*5101-B*5109) of exons 2 and 3 of HLA-B51 gene to determine the 
      HLA-B51 genotypes. The results were subsequently confirmed by (PCR-SSP) and 
      sequencing and were identical to those from polymerase chain reaction-sequencing 
      specific primers (PCR-SSP) in 27 patients with BD and 30 healthy controls. This 
      suggests that we successfully developed the oligochip for Behcet's-associated 
      gene HLA-B51, which can effectively and accurately identify the HLA-B51 
      genotypes.
FAU - Wang, Yun-shan
AU  - Wang YS
AD  - Central Laboratory, Ji'nan Central Hospital, Clinical Medical College of Shandong 
      University, China. Sdjnwys@163.com
FAU - Ma, Xiao-li
AU  - Ma XL
FAU - Sun, Shan-hui
AU  - Sun SH
FAU - Sun, Yue
AU  - Sun Y
FAU - Yu, Bing
AU  - Yu B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genet Test
JT  - Genetic testing
JID - 9802546
RN  - 0 (DNA Probes)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B51 Antigen)
SB  - IM
MH  - *Alleles
MH  - Behcet Syndrome/diagnosis/*genetics
MH  - DNA Mutational Analysis/instrumentation/methods
MH  - DNA Probes/genetics
MH  - Female
MH  - HLA-B Antigens/*genetics
MH  - HLA-B51 Antigen
MH  - Humans
MH  - Lab-On-A-Chip Devices
MH  - Male
MH  - *Microchip Analytical Procedures/methods
MH  - *Oligonucleotide Array Sequence Analysis/instrumentation/methods
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
EDAT- 2005/12/29 09:00
MHDA- 2006/04/13 09:00
CRDT- 2005/12/29 09:00
PHST- 2005/12/29 09:00 [pubmed]
PHST- 2006/04/13 09:00 [medline]
PHST- 2005/12/29 09:00 [entrez]
AID - 10.1089/gte.2005.9.292 [doi]
PST - ppublish
SO  - Genet Test. 2005 Winter;9(4):292-6. doi: 10.1089/gte.2005.9.292.