PMID- 16381641
OWN - NLM
STAT- MEDLINE
DCOM- 20070828
LR  - 20051229
IS  - 1007-3418 (Print)
IS  - 1007-3418 (Linking)
VI  - 13
IP  - 12
DP  - 2005 Dec
TI  - [Inhibition of mouse hepatocyte apoptosis by anti-caspase-12 small interfering 
      RNA].
PG  - 923-6
AB  - OBJECTIVES: To study the inhibition of primary mouse hepatocyte apoptosis by 
      small interfering RNA (siRNAs) against caspase-12. METHODS: The Balb/c mouse 
      primary hepatocytes were isolated in situ with two-step liver perfusion with 0.5 
      g/L collagenase type IV, and apoptosis were induced with 4 micromol/L 
      thapsigargin (TG). The three kingds of siRNAs targeting different gene sites 
      (130, 214, 521) were synthetized chemically. The single-stranded RNAs were 
      annealed to produce double-stranded siRNAs, then the mouse primary hepatocytes 
      were transfected by oligofectamine package. The inhibition of caspase-12 was 
      analyzed with RT-PCR and Western-blot. The viable hepatocytes following the 
      induction of apoptosis were evaluated with MTT. RESULTS: All the three kinds of 
      siRNAs could obviously inhibit normal mouse hepatocyte caspase-12 mRNA. The siRNA 
      (214) were more effective than the other two when the concentration was 100 
      nmol/L. The caspase-12 mRNA expression was inhibited by 52.08%, while that of 
      siRNA (521) was 30.73% (t=4.30, P <0.05). However when the concentration was 200 
      nmol/L, the inhibitions were similar (88.07%, 86.22% and 89.41% respectively). 
      siRNA (214) could downregulate the expression of apoptotic hepatocytes 
      procaspase-12 by 51.43% ( t=4.30, P <0.01). Contrasted with apoptotic 
      hepatocytes, the cell activity, which was analyzed with MTT, increased by 48.76% 
      (t=2.23, P <0.01). CONCLUSION: siRNAs could effectively downregulate the 
      expression of caspase-12 at mRNA and protein levels and prevent mouse primary 
      hepatocytes from apoptosis.
FAU - Liu, Hai-fang
AU  - Liu HF
AD  - Department of Infectious Diseases, Ruijin Hospital, Shanghai Second Medical 
      University, Shanghai 200025, China.
FAU - Xie, Qing
AU  - Xie Q
FAU - Jiang, Shan
AU  - Jiang S
FAU - Li, Guang-ming
AU  - Li GM
FAU - Zhou, Xia-qiu
AU  - Zhou XQ
FAU - Shi, Yi
AU  - Shi Y
FAU - Yu, Hong
AU  - Yu H
FAU - Jin, You-xin
AU  - Jin YX
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Gan Zang Bing Za Zhi
JT  - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of 
      hepatology
JID - 9710009
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - EC 3.4.22.- (Caspase 12)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Caspase 12/*biosynthesis/genetics
MH  - Hepatocytes/*cytology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - RNA, Messenger/biosynthesis/genetics
MH  - RNA, Small Interfering/*genetics
EDAT- 2005/12/31 09:00
MHDA- 2007/08/29 09:00
CRDT- 2005/12/31 09:00
PHST- 2005/12/31 09:00 [pubmed]
PHST- 2007/08/29 09:00 [medline]
PHST- 2005/12/31 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Gan Zang Bing Za Zhi. 2005 Dec;13(12):923-6.