PMID- 16382904
OWN - NLM
STAT- MEDLINE
DCOM- 20060118
LR  - 20220310
IS  - 0736-6205 (Print)
IS  - 0736-6205 (Linking)
VI  - 39
IP  - 6
DP  - 2005 Dec
TI  - Software for quantification of labeled bacteria from digital microscope images by 
      automated image analysis.
PG  - 859-63
AB  - Automated image analysis software, CellC, was developed and validated for 
      quantification of bacterial cells from digital microscope images. CellC enables 
      automated enumeration of bacterial cells, comparison of total count and specific 
      count images [e.g., 4',6-diamino-2-phenylindole (DAPI) and fluorescence in situ 
      hybridization (FISH) images], and provides quantitative estimates of cell 
      morphology. The software includes an intuitive graphical user interface that 
      enables easy usage as well as sequential analysis of multiple images without user 
      intervention. Validation of enumeration reveals correlation to be better than 
      0.98 when total bacterial counts by CellC are compared with manual enumeration, 
      with all validated image types. The software is freely available and modifiable: 
      the executable files and MATLAB source codes can be obtained at www. cs. 
      tut.fi/sgn/csb/cellc.
FAU - Selinummi, Jyrki
AU  - Selinummi J
AD  - Institute of Signal Processing, Tampere University of Technology, Tampere, 
      Finland. jyrki.selinummi@tut.fi
FAU - Seppala, Jenni
AU  - Seppala J
FAU - Yli-Harja, Olli
AU  - Yli-Harja O
FAU - Puhakka, Jaakko A
AU  - Puhakka JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biotechniques
JT  - BioTechniques
JID - 8306785
SB  - IM
MH  - Bioreactors
MH  - Colony Count, Microbial/*methods
MH  - Image Processing, Computer-Assisted/*methods
MH  - Microscopy
MH  - *Software
EDAT- 2005/12/31 09:00
MHDA- 2006/01/19 09:00
CRDT- 2005/12/31 09:00
PHST- 2005/12/31 09:00 [pubmed]
PHST- 2006/01/19 09:00 [medline]
PHST- 2005/12/31 09:00 [entrez]
AID - 000112018 [pii]
AID - 10.2144/000112018 [doi]
PST - ppublish
SO  - Biotechniques. 2005 Dec;39(6):859-63. doi: 10.2144/000112018.