PMID- 16384621
OWN - NLM
STAT- MEDLINE
DCOM- 20060911
LR  - 20061115
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 118
IP  - 1-2
DP  - 2006 Jun
TI  - Antigenic structure analysis of glycosylated protein 3 of porcine reproductive 
      and respiratory syndrome virus.
PG  - 98-104
AB  - The function of the glycosylated protein 3 (GP3), a porcine reproductive and 
      respiratory syndrome virus (PRRSV) associated protein is poorly known. In the 
      present study, the gene encoding GP3 (ORF3), lacking the highly hydrophobic 
      domain in the N- and C-termini was expressed as GST-fusion proteins in E. coli. 
      Monoclonal antibodies (MAbs) against GP3 were developed and used to probe a 
      series of GP3 peptides using ELISA. After precise analysis by sequential deletion 
      of the terminal amino acid residues from each peptide, the minimal epitopes 
      recognized by the MAbs were localized to W(74)CRIGHDRCGED(85) and 
      Y(67)EPGRSLW(74). The epitope sequences were well conserved among most of the 
      North American-type isolates, with the exception of two amino acid mutations in 
      both epitopes in a few of these isolates. Mutational analysis revealed that these 
      mutants were not recognized by any of the five MAbs, indicating that genetic 
      variation could lead to altered antigenicity. Eight out of nine peptide 
      fragments, 58-72aa, 73-87aa, 88-101aa, 102-115aa, 50-65aa, 66-81aa, 80-95aa and 
      94-109aa were recognized by PRRSV-positive pig serum as determined by Western 
      blot analysis. The results herein may elucidate partially the antigenic structure 
      of GP3 and variations of PRRSV.
FAU - Zhou, Yan-Jun
AU  - Zhou YJ
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 
      150001, People's, Republic of China.
FAU - An, Tong-Qing
AU  - An TQ
FAU - He, Yun-Xia
AU  - He YX
FAU - Liu, Jin-Xia
AU  - Liu JX
FAU - Qiu, Hua-Ji
AU  - Qiu HJ
FAU - Wang, Yun-Feng
AU  - Wang YF
FAU - Tong, Guangzhi
AU  - Tong G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051227
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/genetics/*immunology
MH  - Blotting, Western
MH  - Cell Line
MH  - Conserved Sequence
MH  - *Epitope Mapping
MH  - Epitopes, B-Lymphocyte/immunology
MH  - Glycoproteins/genetics/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Porcine Reproductive and Respiratory Syndrome/immunology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine
MH  - Viral Proteins/genetics/*immunology
EDAT- 2005/12/31 09:00
MHDA- 2006/09/12 09:00
CRDT- 2005/12/31 09:00
PHST- 2005/09/20 00:00 [received]
PHST- 2005/11/21 00:00 [revised]
PHST- 2005/11/22 00:00 [accepted]
PHST- 2005/12/31 09:00 [pubmed]
PHST- 2006/09/12 09:00 [medline]
PHST- 2005/12/31 09:00 [entrez]
AID - S0168-1702(05)00358-8 [pii]
AID - 10.1016/j.virusres.2005.11.019 [doi]
PST - ppublish
SO  - Virus Res. 2006 Jun;118(1-2):98-104. doi: 10.1016/j.virusres.2005.11.019. Epub 
      2005 Dec 27.