PMID- 16384928
OWN - NLM
STAT- MEDLINE
DCOM- 20060524
LR  - 20220316
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 107
IP  - 9
DP  - 2006 May 1
TI  - Expansion of CD94/NKG2C+ NK cells in response to human cytomegalovirus-infected 
      fibroblasts.
PG  - 3624-31
AB  - CD94/NKG2C(+) natural killer (NK) cells are increased in healthy individuals 
      infected with human cytomegalovirus (HCMV), suggesting that HCMV infection may 
      shape the NK cell receptor repertoire. To address this question, we analyzed the 
      distribution of NK cell subsets in peripheral blood lymphocytes (PBLs) cocultured 
      with HCMV-infected fibroblasts. A substantial increase of NK cells was detected 
      by day 10 in samples from a group of HCMV(+) donors, and CD94/NKG2C(+) cells 
      outnumbered the CD94/NKG2A(+) subset. Fibroblast infection was required to induce 
      the preferential expansion of CD94/NKG2C(+) NK cells that was comparable with 
      allogeneic or autologous fibroblasts, and different virus strains. A 
      CD94-specific monoclonal antibody (mAb) abrogated the effect, supporting an 
      involvement of the lectinlike receptor. Purified CD56(+) populations stimulated 
      with HCMV-infected cells did not proliferate, but the expansion of the 
      CD94/NKG2C(+) subset was detected in the presence of interleukin-15 (IL-15). 
      Experiments with HCMV deletion mutants indicated that the response of 
      CD94/NKG2C(+) NK cells was independent of the UL16, UL18, and UL40 HCMV genes, 
      but was impaired when cells were infected with a mutant lacking the US2-11 gene 
      region. Taken together the data support that the interaction of CD94/NKG2C with 
      HCMV-infected fibroblasts, concomitant to the inhibition of human leukocyte 
      antigen (HLA) class I expression, promotes an outgrowth of CD94/NKG2C(+) NK 
      cells.
FAU - Guma, Monica
AU  - Guma M
AD  - Molecular Immunopathology Unit, DCEXS, Universitat Pompeu Fabra, Dr Aiguader 80, 
      08003 Barcelona, Spain.
FAU - Budt, Matthias
AU  - Budt M
FAU - Saez, Andrea
AU  - Saez A
FAU - Brckalo, Tamara
AU  - Brckalo T
FAU - Hengel, Hartmut
AU  - Hengel H
FAU - Angulo, Ana
AU  - Angulo A
FAU - Lopez-Botet, Miguel
AU  - Lopez-Botet M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051229
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Interleukin-15)
RN  - 0 (KLRC1 protein, human)
RN  - 0 (KLRC2 protein, human)
RN  - 0 (KLRD1 protein, human)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily C)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily D)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Natural Killer Cell)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Adult
MH  - Cell Line
MH  - Coculture Techniques
MH  - Cytomegalovirus/genetics/*immunology
MH  - Cytomegalovirus Infections/immunology/virology
MH  - Fibroblasts
MH  - Humans
MH  - Interleukin-15/pharmacology
MH  - Killer Cells, Natural/classification/drug effects/*immunology
MH  - Middle Aged
MH  - NK Cell Lectin-Like Receptor Subfamily C
MH  - NK Cell Lectin-Like Receptor Subfamily D/*metabolism
MH  - Receptors, Immunologic/*metabolism
MH  - Receptors, Natural Killer Cell
MH  - Recombinant Proteins/pharmacology
EDAT- 2005/12/31 09:00
MHDA- 2006/05/25 09:00
CRDT- 2005/12/31 09:00
PHST- 2005/12/31 09:00 [pubmed]
PHST- 2006/05/25 09:00 [medline]
PHST- 2005/12/31 09:00 [entrez]
AID - S0006-4971(20)65546-X [pii]
AID - 10.1182/blood-2005-09-3682 [doi]
PST - ppublish
SO  - Blood. 2006 May 1;107(9):3624-31. doi: 10.1182/blood-2005-09-3682. Epub 2005 Dec 
      29.