PMID- 16385820
OWN - NLM
STAT- MEDLINE
DCOM- 20060712
LR  - 20191210
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 67
IP  - 1-2
DP  - 2005 Nov 9
TI  - In vitro research of anti-HSV-1 activity in different extracts from Pacific 
      oysters Crassostrea gigas.
PG  - 141-7
AB  - Mortalities related to the detection of Ostreid Herpesvirus 1 (OsHV-1) have been 
      previously reported in France among larvae and spat of the Pacific oyster 
      Crassostrea gigas. Adult oysters appear less sensitive to herpesvirus infections, 
      although OsHV-1 has been detected in adults without signs of disease or 
      mortality. This suggests that the virus is able to persist in its host and that 
      adult oysters may be able to control OsHV-1 infection. Little is known about 
      antiviral substances in invertebrates. The present work concerns the research of 
      antiviral substances in adult oyster C. gigas, where putative antiviral 
      activities were monitored using 3 strategies: (1) in metabolites with variable 
      polarity, (2) in peptidic extracts and (3) in crude haemolymph. In vitro 
      antiviral assays were based on inhibition of Herpes simplex virus type 1 (HSV-1) 
      replication in Vero cell monolayers. All extracts presented no cytotoxicity. 
      Antiviral activity was detected in the fresh filtered haemolymph (EC50:425 microg 
      ml(-1)) and seasonal variation of the haemolymph antiviral activity was 
      monitored.
FAU - Olicard, Cecile
AU  - Olicard C
AD  - Laboratoire de Biotechnologie et Chimie Marines, EA 2594, Universite de Bretagne 
      Sud, Campus de Tohannic, Centre de recherche Yves Coppens, 56017 Vannes, France.
FAU - Didier, Yohann
AU  - Didier Y
FAU - Marty, Christel
AU  - Marty C
FAU - Bourgougnon, Nathalie
AU  - Bourgougnon N
FAU - Renault, Tristan
AU  - Renault T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (Antiviral Agents)
RN  - 0 (Complex Mixtures)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/blood/metabolism/*pharmacology
MH  - Cell Survival/drug effects
MH  - Chlorocebus aethiops
MH  - Complex Mixtures/blood/metabolism/*pharmacology
MH  - Crassostrea/*chemistry/virology
MH  - Flow Cytometry
MH  - France
MH  - Hemolymph/chemistry
MH  - Herpesvirus 1, Human/*physiology
MH  - Seasons
MH  - Spectrophotometry
MH  - Vero Cells
MH  - Virus Replication/*drug effects/physiology
EDAT- 2006/01/03 09:00
MHDA- 2006/07/13 09:00
CRDT- 2006/01/03 09:00
PHST- 2006/01/03 09:00 [pubmed]
PHST- 2006/07/13 09:00 [medline]
PHST- 2006/01/03 09:00 [entrez]
AID - 10.3354/dao067141 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2005 Nov 9;67(1-2):141-7. doi: 10.3354/dao067141.