PMID- 16386648
OWN - NLM
STAT- MEDLINE
DCOM- 20060922
LR  - 20111117
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 66
IP  - 10
DP  - 2005 Oct
TI  - DRB1-TNF-alpha-TNF-beta haplotype is strongly associated with severe aortoiliac 
      occlusive disease, a clinical form of atherosclerosis.
PG  - 1062-7
AB  - Severe aortoiliac occlusive disease (AOD) is a clinical manifestation of 
      peripheral arteriosclerosis. Atherosclerosis has been associated with some human 
      leukocyte antigen (HLA)-DRB1 alleles, stressing its relationship with autoimmune 
      or inflammatory disorders. Additionally, in rheumatoid arthritis patients, the 
      DRB1*0404 allele is specifically associated with endothelial dysfunction. Our 
      objective was to assess the role of class II HLA alleles in the susceptibility to 
      AOD; a combined study of the nearby tumor necrosis factor (TNF) locus was also 
      performed. We included 104 AOD patients and 504 healthy controls from Madrid. 
      DRB1 typing and DRB1*04 subtyping was done by polymerase chain reaction 
      amplification followed by hybridization with specific oligonucleotides. TNF-alpha 
      and TNF-beta microsatellites were studied by polymerase chain reaction and 
      capillary electrophoresis. None of the markers was associated with AOD, although 
      a trend was observed for DRB1*0404 (OR = 2.18; p = 0.05). However, among 
      DRB1*0404 individuals, the TNFa11-b4 pair was present more frequently in patients 
      than in controls (OR = 16.0; p = 0.007). The combined appearance of TNFa11-b4 and 
      DRB1*0404 was much more frequent in patients than in controls (OR = 5.92; p = 
      0.0013), a result enhanced by haplotypic estimates (OR = 10.0; p = 0.00017). Our 
      results show that the HLA region modulates the predisposition to AOD. More 
      specifically, they suggest that an extended haplotype encompassing DRB1*0404 and 
      TNFa11-b4 carries a genetic factor conferring susceptibility to AOD.
FAU - Mas, Alfonso
AU  - Mas A
AD  - Department of Immunology Hospital Clinico San Carlos, Madrid, Spain.
FAU - Blanco, E
AU  - Blanco E
FAU - Monux, G
AU  - Monux G
FAU - Urcelay, E
AU  - Urcelay E
FAU - Serrano, F J
AU  - Serrano FJ
FAU - de la Concha, E G
AU  - de la Concha EG
FAU - Martinez, A
AU  - Martinez A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051102
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA DRB1*04:04 antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Alleles
MH  - Arterial Occlusive Diseases/*genetics
MH  - Atherosclerosis/*genetics
MH  - Confidence Intervals
MH  - Female
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Haplotypes
MH  - Humans
MH  - Lymphotoxin-alpha/*genetics
MH  - Male
MH  - Middle Aged
MH  - Odds Ratio
MH  - Polymorphism, Genetic
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2006/01/03 09:00
MHDA- 2006/09/23 09:00
CRDT- 2006/01/03 09:00
PHST- 2005/08/10 00:00 [received]
PHST- 2005/09/29 00:00 [accepted]
PHST- 2006/01/03 09:00 [pubmed]
PHST- 2006/09/23 09:00 [medline]
PHST- 2006/01/03 09:00 [entrez]
AID - S0198-8859(05)00425-8 [pii]
AID - 10.1016/j.humimm.2005.10.001 [doi]
PST - ppublish
SO  - Hum Immunol. 2005 Oct;66(10):1062-7. doi: 10.1016/j.humimm.2005.10.001. Epub 2005 
      Nov 2.