PMID- 1638698
OWN - NLM
STAT- MEDLINE
DCOM- 19920828
LR  - 20190510
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 13
IP  - 7
DP  - 1992 Jul
TI  - Temporal changes in the mutant frequency and mutation spectra of the 61st codon 
      of the H-ras oncogene following exposure of B6C3F1 mice to N-nitrosodiethylamine 
      (DEN).
PG  - 1277-9
AB  - Hepatocellular tumors were induced in 15 day old male B6C3F1 mice following a 
      single exposure to N-nitrosodiethylamine (DEN; 5 mg/kg, i.p.). Tumors were 
      collected at 38 and 65 weeks to compare the frequencies and types of mutations in 
      the 61st codon of the H-ras oncogene. The 61st codon was amplified using the 
      polymerase chain reaction (PCR). Allele-specific oligonucleotide (ASO) probes 
      were used to determine the frequency and types of mutations present in these 
      tumors. Forty-nine nodular hepatic lesions were obtained from seven animals at 
      the 38 week timepoint. Five of these samples (10%) had mutations at the 61st 
      codon with one CAA-AAA, one CAA-CGA and three CAA-CTA. Thirty-six nodular hepatic 
      lesions were obtained from six animals at the 65 week timepoint. Ten of these 
      samples (28%) had mutations at the 61st codon with one CAA-AAA, five CAA-CGA and 
      four CAA-CTA. These data indicate that DEN-induced mutations at the 61st codon of 
      the mouse H-ras oncogene (i) are an infrequent event, (ii) have different 
      frequencies at the 38 and 65 week timepoints and (iii) are different from the 
      types of mutations seen in spontaneous lesions.
FAU - Richardson, K K
AU  - Richardson KK
AD  - Toxicology Research Laboratories, Eli Lilly and Company, Greenfield, IN 46140.
FAU - Rexroat, M A
AU  - Rexroat MA
FAU - Helvering, L M
AU  - Helvering LM
FAU - Copple, D M
AU  - Copple DM
FAU - Richardson, F C
AU  - Richardson FC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Codon)
RN  - 0 (Oligonucleotide Probes)
RN  - 3IQ78TTX1A (Diethylnitrosamine)
RN  - 9007-49-2 (DNA)
SB  - IM
GS  - H-ras
MH  - Alleles
MH  - Animals
MH  - Base Sequence
MH  - Codon/drug effects
MH  - DNA/genetics/isolation & purification
MH  - Diethylnitrosamine/*toxicity
MH  - Genes, ras/*drug effects
MH  - Liver/drug effects/pathology
MH  - Liver Neoplasms/*chemically induced/genetics/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - *Mutation
MH  - Oligonucleotide Probes
MH  - Time Factors
EDAT- 1992/07/01 00:00
MHDA- 1992/07/01 00:01
CRDT- 1992/07/01 00:00
PHST- 1992/07/01 00:00 [pubmed]
PHST- 1992/07/01 00:01 [medline]
PHST- 1992/07/01 00:00 [entrez]
AID - 10.1093/carcin/13.7.1277 [doi]
PST - ppublish
SO  - Carcinogenesis. 1992 Jul;13(7):1277-9. doi: 10.1093/carcin/13.7.1277.