PMID- 16388490
OWN - NLM
STAT- MEDLINE
DCOM- 20060330
LR  - 20071114
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 193
IP  - 3
DP  - 2006 Feb 1
TI  - High levels of Epstein-Barr virus DNA in saliva and peripheral blood from Ugandan 
      mother-child pairs.
PG  - 422-6
AB  - In Africa, Epstein-Barr virus (EBV) is associated with Burkitt lymphoma. We 
      measured levels of EBV DNA in saliva and buffy coats from 233 asymptomatic 
      Ugandan children with sickle cell disease and their mothers by quantitative 
      real-time polymerase chain reaction. EBV DNA was detected in saliva from 90% of 
      the children (median [interquartile range [IQR]] level, 5.2 [4.2-6.0] log(10) 
      copies/mL of saliva) and 79% of the mothers (median [IQR] level, 4.8 [3.7-5.6] 
      log(10) copies/mL of saliva) (P < .001). EBV DNA was detected in buffy coats from 
      86% of the children (median [IQR] level, 2.5 [2.2-2.9] log(10) copies/1 x 10(6) 
      peripheral white blood cells [PWBCs]) and 72% of the mothers (median [IQR] level, 
      2.7 [2.4-3.1] log(10) copies/1 x 10(6) PWBCs) (P = .24). Detection of EBV DNA in 
      saliva was positively correlated with detection in buffy coats. EBV DNA was 
      detected more frequently in saliva and buffy coats than was human herpesvirus 8 
      DNA. Our results indicate that EBV infection persists, with virus readily 
      detectable in saliva and buffy coats from persons without apparent symptoms in 
      Africa.
FAU - Mbulaiteye, Sam M
AU  - Mbulaiteye SM
AD  - Division of Cancer Epidemiology and Genetics, National Cancer Institute, 
      Department of Health and Human Services, Rockville, Maryland, USA. 
      mbulaits@mail.nih.gov
FAU - Walters, Michael
AU  - Walters M
FAU - Engels, Eric A
AU  - Engels EA
FAU - Bakaki, Paul M
AU  - Bakaki PM
FAU - Ndugwa, Christopher M
AU  - Ndugwa CM
FAU - Owor, Anchilla M
AU  - Owor AM
FAU - Goedert, James J
AU  - Goedert JJ
FAU - Whitby, Denise
AU  - Whitby D
FAU - Biggar, Robert J
AU  - Biggar RJ
LA  - eng
GR  - N01-C0-12400/PHS HHS/United States
GR  - N02-CP-91027/CP/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20051222
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Anemia, Sickle Cell/complications
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral/*analysis/*blood
MH  - Epstein-Barr Virus Infections/epidemiology/transmission/*virology
MH  - Female
MH  - Herpesvirus 4, Human/genetics/*isolation & purification
MH  - Humans
MH  - Infant
MH  - Prevalence
MH  - Saliva/*chemistry
MH  - Uganda/epidemiology
MH  - Virus Shedding
EDAT- 2006/01/03 09:00
MHDA- 2006/03/31 09:00
CRDT- 2006/01/03 09:00
PHST- 2005/06/09 00:00 [received]
PHST- 2005/08/22 00:00 [accepted]
PHST- 2006/01/03 09:00 [pubmed]
PHST- 2006/03/31 09:00 [medline]
PHST- 2006/01/03 09:00 [entrez]
AID - JID35083 [pii]
AID - 10.1086/499277 [doi]
PST - ppublish
SO  - J Infect Dis. 2006 Feb 1;193(3):422-6. doi: 10.1086/499277. Epub 2005 Dec 22.