PMID- 16388883
OWN - NLM
STAT- MEDLINE
DCOM- 20060309
LR  - 20060123
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 219
IP  - 1-3
DP  - 2006 Feb 15
TI  - Sensitising potential of four textile dyes and some of their metabolites in a 
      modified local lymph node assay.
PG  - 113-23
AB  - We studied the sensitising and allergenic potentials of the textile dyes disperse 
      yellow 3, disperse orange 30, disperse red 82, disperse yellow 211 and two 
      metabolites of disperse yellow 3, 4-aminoacetanilide and 2-amino-p-cresol, using 
      modified protocols of the murine "local lymph node assay" (LLNA). Test substances 
      were applied either to the dorsum of the mice ears (sensitisation protocol) or 
      they were first applied to the skin of their backs and 2 weeks later to their 
      ears (sensitisation-challenge protocol). In addition to the endpoints weight and 
      cell number of the draining ear lymph nodes we analysed lymphocyte subpopulations 
      by flow cytometry. In the sensitisation protocol, disperse yellow 3 and its 
      metabolite 4-aminoacetanilide did not induce significant effects, whereas in the 
      sensitisation-challenge protocol cell number and lymph node weight increased 
      significantly indicating a sensitising potential in NMRI mice. Hence, two-phase 
      treatment (skin of the back, ear) increased the sensitivity of this assay. The 
      second metabolite of disperse yellow 3, 2-amino-p-cresol, showed distinct effects 
      in both treatment protocols; this applied mainly to the parameters cell number 
      and lymph node weight. The dye disperse red 82 caused ambiguous increases in 
      lymph node weight and cell number in the sensitisation protocol which were not 
      reproduced in the sensitisation-challenge protocol, ruling out a relevant 
      sensitising potential for this dye in NMRI mice. Disperse yellow 211 and disperse 
      orange 30 did not induce relevant changes under our experimental conditions. 
      Phenotyping of lymphocytes did not influence the assessment of these dyes.
FAU - Stahlmann, Ralf
AU  - Stahlmann R
AD  - Institute of Clinical Pharmacology and Toxicology, Charite-Campus Benjamin 
      Franklin, Garystrasse 5, 14195 Berlin, Germany. ralf.stahlmann@charite.de
FAU - Wegner, Matthias
AU  - Wegner M
FAU - Riecke, Kai
AU  - Riecke K
FAU - Kruse, Matthias
AU  - Kruse M
FAU - Platzek, Thomas
AU  - Platzek T
LA  - eng
PT  - Journal Article
DEP - 20060104
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Allergens)
RN  - 0 (Azo Compounds)
RN  - 0 (Coloring Agents)
SB  - IM
MH  - Allergens/metabolism/*toxicity
MH  - Animals
MH  - Azo Compounds/*toxicity
MH  - B-Lymphocytes/drug effects
MH  - Coloring Agents/metabolism/*toxicity
MH  - Female
MH  - Flow Cytometry
MH  - Immunohistochemistry
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Phenotype
MH  - Skin/drug effects/metabolism
MH  - T-Lymphocytes/drug effects
MH  - *Textiles
EDAT- 2006/01/04 09:00
MHDA- 2006/03/10 09:00
CRDT- 2006/01/04 09:00
PHST- 2005/09/30 00:00 [received]
PHST- 2005/11/08 00:00 [revised]
PHST- 2005/11/08 00:00 [accepted]
PHST- 2006/01/04 09:00 [pubmed]
PHST- 2006/03/10 09:00 [medline]
PHST- 2006/01/04 09:00 [entrez]
AID - S0300-483X(05)00557-3 [pii]
AID - 10.1016/j.tox.2005.11.005 [doi]
PST - ppublish
SO  - Toxicology. 2006 Feb 15;219(1-3):113-23. doi: 10.1016/j.tox.2005.11.005. Epub 
      2006 Jan 4.