PMID- 16389452
OWN - NLM
STAT- MEDLINE
DCOM- 20060316
LR  - 20111117
IS  - 1420-682X (Print)
IS  - 1420-682X (Linking)
VI  - 63
IP  - 2
DP  - 2006 Jan
TI  - Inhibitors of phosphatidylinositol 3-kinase activity prevent cell cycle 
      progression and induce apoptosis at the M/G1 transition in CHO cells.
PG  - 220-8
AB  - Phosphatidylinositol 3-kinase (PI3-kinase) activity has been implicated in 
      regulating cell cycle progression at distinct points in the cell cycle by 
      preventing cell cycle arrest or apoptosis. In this study, the role of PI3-kinase 
      activity during the entire G1 phase of the ongoing cell cycle was studied in 
      Chinese hamster ovary (CHO) cells synchronized by mitotic shake-off. We show that 
      inhibition of PI3-kinase activity during and 2 h after mitosis inhibited cell 
      cycle progression into S phase. In the presence of the PI3-kinase inhibitor 
      wortmannin or LY294002, cells were arrested during early G1 phase, leading to the 
      expression of the cleaved caspase-3, a central mediator of apoptosis. These 
      results demonstrate that PI3-kinase activity is required for progression through 
      the M/G1 phase. In the absence of PI3-kinase activity, cells are induced for 
      apoptosis in this particular phase of the cell cycle.
FAU - van Opstal, A
AU  - van Opstal A
AD  - Cellular Architecture & Dynamics, Utrecht University, Padualaan 8, 3584 CH, 
      Utrecht, The Netherlands.
FAU - Boonstra, J
AU  - Boonstra J
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Cell Mol Life Sci
JT  - Cellular and molecular life sciences : CMLS
JID - 9705402
RN  - 0 (Protein Kinase Inhibitors)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Actin Cytoskeleton/physiology
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - CHO Cells
MH  - Cell Cycle/*drug effects
MH  - Cricetinae
MH  - Cricetulus
MH  - G1 Phase/*drug effects
MH  - Mitosis
MH  - Phosphatidylinositol 3-Kinases/*physiology
MH  - Protein Kinase Inhibitors/*pharmacology
EDAT- 2006/01/04 09:00
MHDA- 2006/03/17 09:00
CRDT- 2006/01/04 09:00
PHST- 2006/01/04 09:00 [pubmed]
PHST- 2006/03/17 09:00 [medline]
PHST- 2006/01/04 09:00 [entrez]
AID - 10.1007/s00018-005-5412-9 [doi]
PST - ppublish
SO  - Cell Mol Life Sci. 2006 Jan;63(2):220-8. doi: 10.1007/s00018-005-5412-9.