PMID- 16389470
OWN - NLM
STAT- MEDLINE
DCOM- 20060727
LR  - 20181113
IS  - 0957-4530 (Print)
IS  - 0957-4530 (Linking)
VI  - 17
IP  - 1
DP  - 2006 Jan
TI  - Observation and quantitative analysis of rat bone marrow stromal cells cultured 
      in vitro on newly formed transparent beta-tricalcium phosphate.
PG  - 33-41
AB  - To observe living cell morphology on ceramics by light microscopy, we fabricated 
      a new material-transparent beta - tricalcium phosphate (t-beta TCP) ceramic-for 
      the purpose of serving as a tissue culture substrate. Bone marrow stromal cells 
      (BMSCs) were obtained from rat femora and cultured on both t-beta TCP ceramic 
      disks and culture grade polystyrene (PS) dishes in an osteogenic medium. After 1 
      day of culture, cell attachment and spreading on both the t-beta TCP and PS 
      substrata were equally and clearly detected by ordinary light microscopy. After 
      14 days of culture, extensive cell growth, alkaline phosphatase (ALP) staining, 
      and bone mineral deposition could be detected on both substrata. In addition, 
      quantitative biochemical analyses revealed high DNA content, ALP activity, and 
      osteocalcin content of these cultures. This experiment is significant in that all 
      of the results were similarly observed on both the t-beta TCP and PS substrata, 
      indicating the excellent properties of beta TCP ceramics for BMSCs culture 
      towards osteogenic differentiation.
FAU - Kotobuki, Noriko
AU  - Kotobuki N
AD  - Research Institute for Cell Engineering (RICE), National Institute of Advanced 
      Industrial Science and Technology (AIST), 3-11-46 Nakouji, Amagasaki, Hyogo, 
      661-0974, Japan. n.kotobuki@aist.go.jp
FAU - Kawagoe, Daisuke
AU  - Kawagoe D
FAU - Nomura, Daishirou
AU  - Nomura D
FAU - Katou, Youichi
AU  - Katou Y
FAU - Muraki, Kaori
AU  - Muraki K
FAU - Fujimori, Hirotaka
AU  - Fujimori H
FAU - Goto, Seishi
AU  - Goto S
FAU - Ioku, Koji
AU  - Ioku K
FAU - Ohgushi, Hajime
AU  - Ohgushi H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 0 (Biocompatible Materials)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Polystyrenes)
RN  - 0 (beta-tricalcium phosphate)
RN  - 104982-03-8 (Osteocalcin)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Biocompatible Materials/*metabolism
MH  - Bone Marrow Cells/*cytology
MH  - Calcium Phosphates/*metabolism
MH  - Cell Count
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - DNA/analysis
MH  - Femur/cytology
MH  - Male
MH  - Osteocalcin/metabolism
MH  - Polystyrenes/chemistry
MH  - Rats
MH  - Rats, Inbred F344
MH  - Stromal Cells/*cytology/*metabolism
EDAT- 2006/01/04 09:00
MHDA- 2006/07/28 09:00
CRDT- 2006/01/04 09:00
PHST- 2004/08/19 00:00 [received]
PHST- 2005/05/25 00:00 [accepted]
PHST- 2006/01/04 09:00 [pubmed]
PHST- 2006/07/28 09:00 [medline]
PHST- 2006/01/04 09:00 [entrez]
AID - 10.1007/s10856-006-6327-1 [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2006 Jan;17(1):33-41. doi: 10.1007/s10856-006-6327-1.