PMID- 16389639
OWN - NLM
STAT- MEDLINE
DCOM- 20060811
LR  - 20191210
IS  - 1099-498X (Print)
IS  - 1099-498X (Linking)
VI  - 8
IP  - 4
DP  - 2006 Apr
TI  - Protein transduction into human cells by adenovirus dodecahedron using WW domains 
      as universal adaptors.
PG  - 524-31
AB  - BACKGROUND: Direct protein transduction is a recent technique that involves use 
      of peptide vectors. In this study, we demonstrate that adenovirus dodecahedron 
      (Dd), a virus-like particle devoid of DNA and able to penetrate cells with high 
      efficiency, can be used as a vector for protein delivery. METHODS: Taking 
      advantage of Dd interaction with structural domains called WW, we have elaborated 
      a universal adaptor to attach a protein of interest to this vector. RESULTS: A 
      tandem of three WW structural domains derived from the Nedd4 protein enables the 
      formation of stable complexes with Dd, without impairing its endocytosis 
      efficiency. Our protein of interest fused to the triple WW linker is delivered by 
      the dodecahedron in 100% of cells in culture with on average more than ten 
      million molecules per cell. CONCLUSION: These data demonstrate the great 
      potential of adenovirus dodecahedron in combination with WW domains as a protein 
      transduction vector.
CI  - Copyright 2006 John Wiley & Sons, Ltd.
FAU - Garcel, A
AU  - Garcel A
AD  - Institut de Biologie Structurale, 41 rue Jules Horowitz, 38027 Grenoble, France.
FAU - Gout, E
AU  - Gout E
FAU - Timmins, J
AU  - Timmins J
FAU - Chroboczek, J
AU  - Chroboczek J
FAU - Fender, P
AU  - Fender P
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gene Med
JT  - The journal of gene medicine
JID - 9815764
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (Peptide Fragments)
RN  - 0 (Proteins)
RN  - EC 2.3.2.26 (Nedd4 Ubiquitin Protein Ligases)
RN  - EC 2.3.2.26 (Nedd4 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Biological Transport
MH  - Cloning, Molecular
MH  - Drug Delivery Systems
MH  - Drug Design
MH  - Endosomal Sorting Complexes Required for Transport
MH  - HeLa Cells
MH  - Humans
MH  - Nedd4 Ubiquitin Protein Ligases
MH  - Peptide Fragments/*therapeutic use
MH  - Proteins/*genetics
MH  - Ubiquitin-Protein Ligases/genetics
EDAT- 2006/01/04 09:00
MHDA- 2006/08/12 09:00
CRDT- 2006/01/04 09:00
PHST- 2006/01/04 09:00 [pubmed]
PHST- 2006/08/12 09:00 [medline]
PHST- 2006/01/04 09:00 [entrez]
AID - 10.1002/jgm.862 [doi]
PST - ppublish
SO  - J Gene Med. 2006 Apr;8(4):524-31. doi: 10.1002/jgm.862.