PMID- 16390828
OWN - NLM
STAT- MEDLINE
DCOM- 20060316
LR  - 20211203
IS  - 1071-5762 (Print)
IS  - 1029-2470 (Linking)
VI  - 40
IP  - 2
DP  - 2006 Feb
TI  - Aging influences multiple incidices of oxidative stress in the aortic media of 
      the Fischer 344/NNiaxBrown Norway/BiNia rat.
PG  - 185-97
AB  - Here, we determine the influence of aging on multiple markers of oxidative stress 
      in the aorta of adult (6-month), aged (30-month) and very aged (36-month) Fischer 
      344/NNiaHSdxBrown Norway/BiNia (F344/NxBN) rats. Compared to adults, increases in 
      as determined by oxidation of hydroethidine (HE) to ethidium (Et) were increased 
      79.7+/-7.0% in 36-month aortae and this finding was highly correlated with 
      increases in medal thickness (r=0.773, p<0.01) and total protein nitration 
      (r=0.706, p<0.01) but not Ki67, a marker for cell proliferation. Regression 
      analysis showed that increases in aortic superoxide anion (O.-2) with aging were 
      significantly correlated with changes in the expression and/or regulation of 
      proteins involved in metabolic (AMPK-alpha), signaling (mitogen activated protein 
      kinases (MAPKs) along with c-Src), apoptotic (Bax, Bcl-2, Traf-2) and 
      transcriptional (NF-kappaB) activities. These results suggest that the aging 
      F344/NxBN aorta may be highly suited for unraveling the molecular events that 
      lead to age-associated alterations in aortic oxidative stress.
FAU - Rice, K M
AU  - Rice KM
AD  - Marshall University, Department of Biological Sciences, Huntington, WV 2755-1090, 
      USA.
FAU - Preston, D L
AU  - Preston DL
FAU - Walker, E M
AU  - Walker EM
FAU - Blough, E R
AU  - Blough ER
LA  - eng
GR  - AG-20370/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (NF-kappa B)
RN  - 0 (Phenanthridines)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 38483-26-0 (hydroethidine)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EN464416SI (Ethidium)
SB  - IM
MH  - AMP-Activated Protein Kinases
MH  - Aging/*physiology
MH  - Animals
MH  - Aorta/*metabolism
MH  - Cell Proliferation
MH  - Ethidium/chemistry
MH  - Genes, src
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Ki-67 Antigen/metabolism
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Multienzyme Complexes
MH  - NF-kappa B/genetics/metabolism
MH  - *Oxidative Stress
MH  - Phenanthridines/chemistry
MH  - Phosphorylation
MH  - Protein Serine-Threonine Kinases
MH  - Proteins/chemistry/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Inbred F344
MH  - TNF Receptor-Associated Factor 2/metabolism
MH  - bcl-2-Associated X Protein/metabolism
EDAT- 2006/01/05 09:00
MHDA- 2006/03/17 09:00
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/03/17 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
AID - W915U8G6HG71368N [pii]
AID - 10.1080/10715760500464957 [doi]
PST - ppublish
SO  - Free Radic Res. 2006 Feb;40(2):185-97. doi: 10.1080/10715760500464957.