PMID- 16390831
OWN - NLM
STAT- MEDLINE
DCOM- 20060316
LR  - 20181203
IS  - 1071-5762 (Print)
IS  - 1029-2470 (Linking)
VI  - 40
IP  - 2
DP  - 2006 Feb
TI  - Preventive effects of phosphorylated ascorbate on ultraviolet-B induced apoptotic 
      cell death and DNA strand cleavage through enrichment of intracellular vitamin C 
      in skin epidermal keratinocytes.
PG  - 213-21
AB  - Mortality of mouse keratinocytes Pam212 that were irradiated with ultraviolet-B 
      (UVB) was shown to be repressed by pre-irradiated administration with L-ascorbic 
      acid (Asc) or more markedly with Asc-2-O-phosphate (Asc2P), but not with 
      dehydroascorbic acid (DehAsc) or Asc-2-O-alpha-glucoside (Asc2G), although not 
      repressed by post-irradiated administration. The cytoprotection by Asc2P was 
      restricted against UVB below 5-20 mJ/cm2, and exhibited markedly by 
      administration for a period over 2 h, which may be caused by intracellular Asc 
      that was accumulated via dephosphorylation of Asc2P and was increased, 6-24 h 
      after, to levels above twice as abundant as those of Asc-administration. 
      Pre-irradiated Asc2P-administration slightly repressed a DNA ladder-like 
      electrophoretic pattern for UVB-irradiated keratinocytes, containing the 
      histone-bound DNA fragments as shown by ELISA assay, and appreciably repressed 
      the DNA-3'OH cleavage terminals as shown by terminal deoxyribonucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) stain. Thus, prevention of 
      UVB-induced cell death by Asc2P was shown to occur concurrently with inhibition 
      of DNA cleavages and enrichment of intracellular Asc.
FAU - Sugimoto, Miho
AU  - Sugimoto M
AD  - Prefectural University of Hiroshima, Laboratory of Cell-Death Control 
      BioTechnology, Faculty of Life and Environmental Sciences, Shobara, Hiroshima, 
      727-0023, Japan.
FAU - Okugawa, Yoji
AU  - Okugawa Y
FAU - Miwa, Nobuhiko
AU  - Miwa N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 23313-12-4 (ascorbate-2-phosphate)
RN  - 9007-49-2 (DNA)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/*radiation effects
MH  - Ascorbic Acid/*analogs & derivatives/*metabolism/pharmacology
MH  - Cells, Cultured
MH  - Cytoprotection
MH  - DNA/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epidermal Cells
MH  - Epidermis/drug effects/radiation effects
MH  - In Situ Nick-End Labeling
MH  - Keratinocytes/drug effects/*metabolism/radiation effects
MH  - Mice
MH  - Phosphorylation
MH  - Skin/cytology/drug effects/radiation effects
MH  - Ultraviolet Rays
EDAT- 2006/01/05 09:00
MHDA- 2006/03/17 09:00
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/03/17 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
AID - L50282G724L68775 [pii]
AID - 10.1080/10715760500417005 [doi]
PST - ppublish
SO  - Free Radic Res. 2006 Feb;40(2):213-21. doi: 10.1080/10715760500417005.