PMID- 16390958
OWN - NLM
STAT- MEDLINE
DCOM- 20060321
LR  - 20220331
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 44
IP  - 1
DP  - 2006 Jan
TI  - Faster identification of pathogens in positive blood cultures by fluorescence in 
      situ hybridization in routine practice.
PG  - 119-23
AB  - Rapid identification of microorganisms in blood cultures is required to optimize 
      empirical treatment at an early stage. Fluorescence in situ hybridization (FISH) 
      can reduce the time to identification of microorganisms in growth-positive blood 
      cultures. In this study, we evaluated the performance, time to identification, 
      and potential clinical benefits of FISH compared to those of conventional culture 
      methods in routine practice. After Gram staining, blood culture fluids were 
      simultaneously further identified with FISH and with conventional culture 
      methods. Results and points in time of FISH and culture identification 
      (provisional and final identifications) were collected and compared. For 91% of 
      microorganisms, the genus or family name was identified, and for 79%, the species 
      name could be attributed. The sensitivity and specificity of the individual 
      probes exceeded 95%, except for the Enterobacteriaceae probe (sensitivity, 89%). 
      Cross-hybridization was obtained with the Klebsiella pneumoniae probe for 
      Klebsiella oxytoca. The time gains of FISH and final culture identification were 
      more than 18 h for bacteria and 42 h for yeasts. With FISH, Staphylococcus aureus 
      was differentiated from coagulase-negative staphylococci 1.4 h faster than by 
      provisional identification (P < 0.001). In conclusion, FISH allows rapid and 
      reliable identification of the majority of microorganisms in growth-positive 
      blood cultures. The substantial time gain of identification with FISH may allow 
      same-day adjustment of antimicrobial therapy, and FISH is especially useful if no 
      provisional identification is obtained. With further extension of the number of 
      probes and a reduction in turnaround time, FISH will become a very useful 
      diagnostic tool in the diagnosis of bloodstream infections.
FAU - Peters, Remco P H
AU  - Peters RP
AD  - Department of Internal Medicine, VU University Medical Center, Amsterdam, The 
      Netherlands.
FAU - Savelkoul, Paul H M
AU  - Savelkoul PH
FAU - Simoons-Smit, Alberdina M
AU  - Simoons-Smit AM
FAU - Danner, Sven A
AU  - Danner SA
FAU - Vandenbroucke-Grauls, Christina M J E
AU  - Vandenbroucke-Grauls CM
FAU - van Agtmael, Michiel A
AU  - van Agtmael MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Culture Media)
SB  - IM
MH  - Bacteremia/*microbiology
MH  - Bacteria/classification/growth & development/*isolation & purification
MH  - Bacteriological Techniques/*methods
MH  - Blood
MH  - Clinical Protocols
MH  - Culture Media
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Sensitivity and Specificity
PMC - PMC1351964
EDAT- 2006/01/05 09:00
MHDA- 2006/03/22 09:00
PMCR- 2006/05/01
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/03/22 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
PHST- 2006/05/01 00:00 [pmc-release]
AID - 44/1/119 [pii]
AID - 1669-05 [pii]
AID - 10.1128/JCM.44.1.119-123.2006 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2006 Jan;44(1):119-23. doi: 10.1128/JCM.44.1.119-123.2006.