PMID- 16391015
OWN - NLM
STAT- MEDLINE
DCOM- 20060524
LR  - 20210526
IS  - 0006-4971 (Print)
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 107
IP  - 9
DP  - 2006 May 1
TI  - The PANE1 gene encodes a novel human minor histocompatibility antigen that is 
      selectively expressed in B-lymphoid cells and B-CLL.
PG  - 3779-86
AB  - Minor histocompatibility antigens (mHAg's) are peptides encoded by polymorphic 
      genes that are presented by major histocompatibility complex (MHC) molecules and 
      recognized by T cells in recipients of allogeneic hematopoietic cell transplants. 
      Here we report that an alternative transcript of the proliferation-associated 
      nuclear element 1 (PANE1) gene encodes a novel human leukocyte antigen 
      (HLA)-A(*)0301-restricted mHAg that is selectively expressed in B-lymphoid cells. 
      The antigenic peptide is entirely encoded within a unique exon not present in 
      other PANE1 transcripts. Sequencing of PANE1 alleles in mHAg-positive and 
      mHAg-negative cells demonstrates that differential T-cell recognition is due to a 
      single nucleotide polymorphism within the variant exon that replaces an arginine 
      codon with a translation termination codon. The PANE1 transcript that encodes the 
      mHAg is expressed at high levels in resting CD19(+) B cells and B-lineage chronic 
      lymphocytic leukemia (B-CLL) cells, and at significantly lower levels in 
      activated B cells. Activation of B-CLL cells through CD40 ligand (CD40L) 
      stimulation decreases expression of the mHAg-encoding PANE1 transcript and 
      reciprocally increases expression of PANE1 transcripts lacking the mHAg-encoding 
      exon. These studies suggest distinct roles for different PANE1 isoforms in 
      resting compared with activated CD19(+) cells, and identify PANE1 as a potential 
      therapeutic target in B-CLL.
FAU - Brickner, Anthony G
AU  - Brickner AG
AD  - Department of Medicine, Unviersity of Pittsburgh School of Medicine, University 
      of Pittsburgh Cancer Institute, PA, USA.
FAU - Evans, Anne M
AU  - Evans AM
FAU - Mito, Jeffrey K
AU  - Mito JK
FAU - Xuereb, Suzanne M
AU  - Xuereb SM
FAU - Feng, Xin
AU  - Feng X
FAU - Nishida, Tetsuya
AU  - Nishida T
FAU - Fairfull, Liane
AU  - Fairfull L
FAU - Ferrell, Robert E
AU  - Ferrell RE
FAU - Foon, Kenneth A
AU  - Foon KA
FAU - Hunt, Donald F
AU  - Hunt DF
FAU - Shabanowitz, Jeffrey
AU  - Shabanowitz J
FAU - Engelhard, Victor H
AU  - Engelhard VH
FAU - Riddell, Stanley R
AU  - Riddell SR
FAU - Warren, Edus H
AU  - Warren EH
LA  - eng
GR  - CA18029/CA/NCI NIH HHS/United States
GR  - AI339933/AI/NIAID NIH HHS/United States
GR  - AI44134/AI/NIAID NIH HHS/United States
GR  - AI20963/AI/NIAID NIH HHS/United States
GR  - CA106512/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060103
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD19)
RN  - 0 (CENPM protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Epitopes)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*03:01 antigen)
RN  - 0 (HLA-A3 Antigen)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Nuclear Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Antigens, CD19/metabolism
MH  - B-Lymphocytes/*immunology
MH  - Base Sequence
MH  - Cell Cycle Proteins
MH  - DNA/genetics
MH  - Epitopes/chemistry
MH  - Gene Expression
MH  - HLA-A Antigens/genetics
MH  - HLA-A3 Antigen
MH  - Humans
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/*immunology
MH  - Lymphocyte Activation
MH  - Minor Histocompatibility Antigens/chemistry/*genetics
MH  - *Minor Histocompatibility Loci
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/chemistry/*genetics/*immunology
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - T-Lymphocytes, Cytotoxic/immunology
PMC - PMC1895781
EDAT- 2006/01/05 09:00
MHDA- 2006/05/25 09:00
PMCR- 2007/05/01
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/05/25 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
PHST- 2007/05/01 00:00 [pmc-release]
AID - S0006-4971(20)65568-9 [pii]
AID - 3779 [pii]
AID - 10.1182/blood-2005-08-3501 [doi]
PST - ppublish
SO  - Blood. 2006 May 1;107(9):3779-86. doi: 10.1182/blood-2005-08-3501. Epub 2006 Jan 
      3.