PMID- 16391119
OWN - NLM
STAT- MEDLINE
DCOM- 20060301
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 72
IP  - 1
DP  - 2006 Jan
TI  - Modulation of rat cecal microbiota by administration of raffinose and 
      encapsulated Bifidobacterium breve.
PG  - 784-92
AB  - To investigate the effects of administration of raffinose and encapsulated 
      Bifidobacterium breve JCM 1192T cells on the rat cecal microbiota, in a 
      preclinical synbiotic study groups of male WKAH/Hkm Slc rats were fed for 3 weeks 
      with four different test diets: basal diet (group BD), basal diet supplemented 
      with raffinose (group RAF), basal diet supplemented with encapsulated B. breve 
      (group CB), and basal diet supplemented with both raffinose and encapsulated B. 
      breve (group RCB). The bacterial populations in cecal samples were determined by 
      fluorescence in situ hybridization (FISH) and terminal restriction fragment 
      length polymorphism (T-RFLP). B. breve cells were detected only in the RCB group 
      and accounted for about 6.3% of the total cells as determined by FISH analysis. 
      B. breve was also detected only in the RCB group by T-RFLP analysis. This was in 
      contrast to the CB group, in which no B. breve signals were detected by either 
      FISH or T-RFLP. Increases in the sizes of the populations of Bifidobacterium 
      animalis, a Bifidobacterium indigenous to the rat, were observed in the RAF and 
      RCB groups. Principal-component analysis of T-RFLP results revealed significant 
      alterations in the bacterial populations of rats in the RAF and RCB groups; the 
      population in the CB group was similar to that in the control group (group BD). 
      To the best of our knowledge, these results provide the first clear picture of 
      the changes in the rat cecal microbiota in response to synbiotic administration.
FAU - Dinoto, Achmad
AU  - Dinoto A
AD  - Laboratory of Microbial Resources and Ecology, Graduate School of Agriculture, 
      Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
FAU - Suksomcheep, Akarat
AU  - Suksomcheep A
FAU - Ishizuka, Satoshi
AU  - Ishizuka S
FAU - Kimura, Hanae
AU  - Kimura H
FAU - Hanada, Satoshi
AU  - Hanada S
FAU - Kamagata, Yoichi
AU  - Kamagata Y
FAU - Asano, Kozo
AU  - Asano K
FAU - Tomita, Fusao
AU  - Tomita F
FAU - Yokota, Atsushi
AU  - Yokota A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Oligonucleotide Probes)
RN  - N5O3QU595M (Raffinose)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Bacteria/classification/genetics/*isolation & purification
MH  - Bifidobacterium/genetics/*growth & development/isolation & purification
MH  - Cecum/*microbiology
MH  - *Dietary Supplements
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Oligonucleotide Probes
MH  - Polymorphism, Restriction Fragment Length
MH  - Raffinose/*administration & dosage
MH  - Rats
PMC - PMC1352276
EDAT- 2006/01/05 09:00
MHDA- 2006/03/02 09:00
PMCR- 2006/05/01
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/03/02 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
PHST- 2006/05/01 00:00 [pmc-release]
AID - 72/1/784 [pii]
AID - 1790-05 [pii]
AID - 10.1128/AEM.72.1.784-792.2006 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2006 Jan;72(1):784-92. doi: 
      10.1128/AEM.72.1.784-792.2006.