PMID- 16391126
OWN - NLM
STAT- MEDLINE
DCOM- 20060301
LR  - 20191210
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 72
IP  - 1
DP  - 2006 Jan
TI  - Fluorescence in situ hybridization using peptide nucleic acid probes for rapid 
      detection of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. 
      paratuberculosis in potable-water biofilms.
PG  - 848-53
AB  - Here, we present for the first time a high-affinity peptide nucleic acid (PNA) 
      oligonucleotide sequence for detecting Mycobacterium avium bacteria, including 
      the opportunistically pathogenic subspecies M. avium subsp. avium, M. avium 
      subsp. paratuberculosis, and M. avium subsp. silvaticum, by the fluorescence in 
      situ hybridization (FISH) method. There is evidence that M. avium subsp. avium 
      especially is able to survive and grow in drinking-water biofilms and possibly 
      transmit via drinking water. The designed PNA probe (MAV148) specificity was 
      tested with several bacterial species, including other mycobacteria and mycolic 
      acid-containing bacteria. From the range of bacterial strains tested, only M. 
      avium subsp. avium and M. avium subsp. paratuberculosis strains were hybridized. 
      The PNA FISH method was applied successfully to detect M. avium subsp. avium 
      spiked in water samples and biofilm established within a Propella biofilm reactor 
      fed with potable water from a distribution supply.
FAU - Lehtola, Markku J
AU  - Lehtola MJ
AD  - Environmental Healthcare Unit, School of Biological Sciences, University of 
      Southampton, Southampton SO16 7PX, United Kingdom. Markku.Lehtola@ktl.fi
FAU - Torvinen, Eila
AU  - Torvinen E
FAU - Miettinen, Ilkka T
AU  - Miettinen IT
FAU - Keevil, C William
AU  - Keevil CW
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Peptide Nucleic Acids)
SB  - IM
MH  - Biofilms/*growth & development
MH  - Bioreactors
MH  - Fresh Water/*microbiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mycobacterium avium/genetics/*isolation & purification
MH  - Mycobacterium avium subsp. paratuberculosis/genetics/*isolation & purification
MH  - Oligonucleotide Probes/genetics
MH  - Peptide Nucleic Acids/*genetics
MH  - Sensitivity and Specificity
MH  - Species Specificity
MH  - Time Factors
MH  - Water Supply
PMC - PMC1352208
EDAT- 2006/01/05 09:00
MHDA- 2006/03/02 09:00
PMCR- 2006/05/01
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/03/02 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
PHST- 2006/05/01 00:00 [pmc-release]
AID - 72/1/848 [pii]
AID - 1259-05 [pii]
AID - 10.1128/AEM.72.1.848-853.2006 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2006 Jan;72(1):848-53. doi: 
      10.1128/AEM.72.1.848-853.2006.