PMID- 16391135
OWN - NLM
STAT- MEDLINE
DCOM- 20060301
LR  - 20191210
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 72
IP  - 1
DP  - 2006 Jan
TI  - Catalyzed reporter deposition-fluorescence in situ hybridization allows for 
      enrichment-independent detection of microcolony-forming soil bacteria.
PG  - 918-22
AB  - Advances in the growth of hitherto unculturable soil bacteria have emphasized the 
      requirement for rapid bacterial identification methods. Due to the slow-growing 
      strategy of microcolony-forming soil bacteria, successful fluorescence in situ 
      hybridization (FISH) requires an rRNA enrichment step for visualization. In this 
      study, catalyzed reporter deposition (CARD)-FISH was employed as an alternative 
      method to rRNA enhancement and was found to be superior to conventional FISH for 
      the detection of microcolonies that are cultivated by using the soil substrate 
      membrane system. CARD-FISH enabled real-time identification of oligophilic 
      microcolony-forming soil bacteria without the requirement for enrichment on 
      complex media and the associated shifts in community composition.
FAU - Ferrari, Belinda C
AU  - Ferrari BC
AD  - Department of Chemistry and Biomolecular Sciences, Division of Environmental and 
      Life Sciences, Macquarie University, Sydney, NSW 2109, Australia. 
      bferrari@rna.bio.mq.edu.au
FAU - Tujula, Niina
AU  - Tujula N
FAU - Stoner, Kate
AU  - Stoner K
FAU - Kjelleberg, Staffan
AU  - Kjelleberg S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Culture Media)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Polycarboxylate Cement)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal)
RN  - 25766-59-0 (polycarbonate)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - Bacteria/*classification/genetics/growth & development/*isolation & purification
MH  - Bacterial Typing Techniques
MH  - Colony Count, Microbial
MH  - Culture Media
MH  - Horseradish Peroxidase
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Membranes, Artificial
MH  - Polycarboxylate Cement
MH  - RNA, Bacterial
MH  - RNA, Ribosomal
MH  - *Soil Microbiology
PMC - PMC1352241
EDAT- 2006/01/05 09:00
MHDA- 2006/03/02 09:00
PMCR- 2006/05/01
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/03/02 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
PHST- 2006/05/01 00:00 [pmc-release]
AID - 72/1/918 [pii]
AID - 1538-05 [pii]
AID - 10.1128/AEM.72.1.918-922.2006 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2006 Jan;72(1):918-22. doi: 
      10.1128/AEM.72.1.918-922.2006.