PMID- 16391493
OWN - NLM
STAT- MEDLINE
DCOM- 20060717
LR  - 20131121
IS  - 0030-3747 (Print)
IS  - 0030-3747 (Linking)
VI  - 38
IP  - 3
DP  - 2006
TI  - Effect of berberine on interleukin 8 and monocyte chemotactic protein 1 
      expression in a human retinal pigment epithelial cell line.
PG  - 149-57
AB  - PURPOSE: The aims of this study were to examine the effects of berberine, an 
      alkaloid isolated from some medicinal herbs, on interleukin 8 (IL-8) and monocyte 
      chemotactic protein 1 (MCP-1) expression in a human retinal pigment epithelial 
      cell line (ARPE-19) stimulated with interleukin 1beta (IL-1beta) or tumor 
      necrosis factor alpha (TNF-alpha). METHODS: ARPE-19 cells were cultured to 
      confluence. Berberine and IL-1beta or TNF-alpha were added to the medium. IL-8 
      mRNA and MCP-1 mRNA were measured by semiquantitative reverse-transcription 
      polymerase chain reaction and real-time polymerase chain reaction. IL-8 and MCP-1 
      protein concentrations in the media were measured using enzyme-linked 
      immunosorbent assay. RESULTS: Berberine dose-dependently inhibited IL-8 mRNA and 
      MCP-1 mRNA expression of the cells and protein levels in the media stimulated 
      with IL-1beta or TNF-alpha. CONCLUSION: These findings indicate that berberine 
      dose-dependently inhibited the expression of IL-8 and MCP-1 induced by IL-1beta 
      or TNF-alpha.
CI  - Copyright (c) 2006 S. Karger AG, Basel.
FAU - Cui, Hu-Shan
AU  - Cui HS
AD  - Department of Ophthalmology, Toyama Medical and Pharmaceutical University, 
      Toyama, Japan. ophthal@ms.toyama-mpu.ac.jp
FAU - Hayasaka, Seiji
AU  - Hayasaka S
FAU - Zhang, Xue-Yun
AU  - Zhang XY
FAU - Hayasaka, Yoriko
AU  - Hayasaka Y
FAU - Chi, Zai-Long
AU  - Chi ZL
FAU - Zheng, Lian-Shun
AU  - Zheng LS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060102
PL  - Switzerland
TA  - Ophthalmic Res
JT  - Ophthalmic research
JID - 0267442
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0I8Y3P32UF (Berberine)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Berberine/*pharmacology
MH  - Cell Line
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Interleukin-8/*genetics/metabolism
MH  - Pigment Epithelium of Eye/*drug effects/metabolism
MH  - RNA/isolation & purification
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2006/01/05 09:00
MHDA- 2006/07/18 09:00
CRDT- 2006/01/05 09:00
PHST- 2005/08/10 00:00 [received]
PHST- 2005/11/21 00:00 [accepted]
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/07/18 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
AID - 90672 [pii]
AID - 10.1159/000090672 [doi]
PST - ppublish
SO  - Ophthalmic Res. 2006;38(3):149-57. doi: 10.1159/000090672. Epub 2006 Jan 2.