PMID- 16391519
OWN - NLM
STAT- MEDLINE
DCOM- 20060201
LR  - 20211203
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 37
IP  - 6
DP  - 2005 Dec 31
TI  - Downstream components of RhoA required for signal pathway of superoxide formation 
      during phagocytosis of serum opsonized zymosans in macrophages.
PG  - 575-87
AB  - Rac1 and Rac2 are essential for the control of oxidative burst catalyzed by NADPH 
      oxidase. It was also documented that Rho is associated with the superoxide burst 
      reaction during phagocytosis of serum- (SOZ) and IgG-opsonized zymosan particles 
      (IOZ). In this study, we attempted to reveal the signal pathway components in the 
      superoxide formation regulated by Rho GTPase. Tat-C3 blocked superoxide 
      production, suggesting that RhoA is essentially involved in superoxide formation 
      during phagocytosis of SOZ. Conversely SOZ activated both RhoA and Rac1/2. 
      Inhibition of RhoA-activated kinase (ROCK), an important downstream effector of 
      RhoA, by Y27632 and myosin light chain kinase (MLCK) by ML-7 abrogated superoxide 
      production by SOZ. Extracellular signaling-regulated kinase (ERK)1/2 and p38 
      mitogen-activated protein kinase (MAPK) were activated during phagocytosis of 
      SOZ, and Tat-C3 and SB203580 reduced ERK1/2 and p38 MAPK activation, suggesting 
      that RhoA and p38 MAPK may be upstream regulators of ERK1/2. Inhibition of 
      ERK1/2, p38 MAPK, phosphatidyl inositol 3-kinase did not block translocation of 
      RhoA to membranes, suggesting that RhoA is upstream to these kinases. Inhibition 
      of RhoA by Tat-C3 blocked phosphorylation of p47(PHOX). Taken together, RhoA, 
      ROCK, p38MAPK, ERK1/2, and p47(PHOX) may be subsequently activated, leading to 
      activation of NADPH oxidase to produce superoxide.
FAU - Kim, Jun Sub
AU  - Kim JS
AD  - Department of Biochemistry, College of Medicine, Hallym University, Chuncheon 
      220-702, Korea.
FAU - Kim, Jae Gyu
AU  - Kim JG
FAU - Jeon, Chan Young
AU  - Jeon CY
FAU - Won, Ha Young
AU  - Won HY
FAU - Moon, Mi Young
AU  - Moon MY
FAU - Seo, Ji Yeon
AU  - Seo JY
FAU - Kim, Jong Il
AU  - Kim JI
FAU - Kim, Jaebong
AU  - Kim J
FAU - Lee, Jae Yong
AU  - Lee JY
FAU - Choi, Soo Young
AU  - Choi SY
FAU - Park, Jinseu
AU  - Park J
FAU - Yoon Park, Jung Han
AU  - Yoon Park JH
FAU - Ha, Kwon Soo
AU  - Ha KS
FAU - Kim, Pyeung Hyeun
AU  - Kim PH
FAU - Park, Jae Bong
AU  - Park JB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Macrophage-1 Antigen)
RN  - 0 (Opsonin Proteins)
RN  - 11062-77-4 (Superoxides)
RN  - 9010-72-4 (Zymosan)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
RN  - EC 2.7.11.18 (Myosin-Light-Chain Kinase)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Membrane
MH  - Cytosol
MH  - Enzyme Inhibitors/pharmacology
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Macrophage-1 Antigen/pharmacology
MH  - Macrophages/drug effects/*metabolism/ultrastructure
MH  - Mice
MH  - Myosin-Light-Chain Kinase/metabolism
MH  - Opsonin Proteins/blood/*metabolism
MH  - *Phagocytosis
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Protein Transport
MH  - *Signal Transduction
MH  - Superoxides/*metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Zymosan/*blood
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
MH  - rho-Associated Kinases
MH  - rhoA GTP-Binding Protein/antagonists & inhibitors/*metabolism
EDAT- 2006/01/05 09:00
MHDA- 2006/02/02 09:00
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/02/02 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
AID - 200512318 [pii]
AID - 10.1038/emm.2005.71 [doi]
PST - ppublish
SO  - Exp Mol Med. 2005 Dec 31;37(6):575-87. doi: 10.1038/emm.2005.71.