PMID- 16391676
OWN - NLM
STAT- MEDLINE
DCOM- 20070308
LR  - 20220321
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 48
IP  - 6
DP  - 2005 Dec
TI  - Molecular cytogenetic characterization of Aegilops biuncialis and its use for the 
      identification of 5 derived wheat-Aegilops biuncialis disomic addition lines.
PG  - 1070-82
AB  - The aim of the experiments was to produce and identify different Triticum 
      aestivum-Aegilops biuncialis disomic addition lines. To facilitate the exact 
      identification of the Ae. biuncialis chromosomes in these Triticum aestivum-Ae. 
      biuncialis disomic additions, it was necessary to analyze the fluorescence in 
      situ hybridization (FISH) pattern of Ae. biuncialis (2n = 4x = 28, 
      U(b)U(b)M(b)M(b)), comparing it with the diploid progenitors (Aegilops 
      umbellulata, 2n = 2x = 14, UU and Aegilops comosa, 2n = 2x = 14, MM). To identify 
      the Ae. biuncialis chromosomes, FISH was carried out using 2 DNA clones (pSc119.2 
      and pAs1) on Ae. biuncialis and its 2 diploid progenitor species. Differences in 
      the hybridization patterns of all chromosomes were observed among the 4 Ae. 
      umbellulata accessions, the 4 Ae. comosa accessions, and the 3 Ae. biuncialis 
      accessions analyzed. The hybridization pattern of the M genome was more variable 
      than that of the U genome. Five different wheat-Ae. biuncialis addition lines 
      were produced from the wheat-Ae. biuncialis amphiploids produced earlier in 
      Martonvasar. The 2M, 3M, 7M, 3U, and 5U chromosome pairs were identified with 
      FISH using 3 repetitive DNA clones (pSc119.2, pAs1, and pTa71) in the disomic 
      chromosome additions produced. Genomic in situ hybridization (GISH) was used to 
      differentiate the Ae. biuncialis chromosomes from wheat, but no chromosome 
      rearrangements between wheat and Ae. biuncialis were detected in the addition 
      lines.
FAU - Schneider, Annamaria
AU  - Schneider A
AD  - Agricultural Research Institute of the Hungarian Academy of Sciences, 
      Martonvasar.
FAU - Linc, Gabriella
AU  - Linc G
FAU - Molnar, Istvan
AU  - Molnar I
FAU - Molnar-Lang, Marta
AU  - Molnar-Lang M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
MH  - *Cytogenetic Analysis
MH  - *Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Poaceae/anatomy & histology/classification/*genetics
MH  - Triticum/*genetics
EDAT- 2006/01/05 09:00
MHDA- 2007/03/09 09:00
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2007/03/09 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
AID - g05-062 [pii]
AID - 10.1139/g05-062 [doi]
PST - ppublish
SO  - Genome. 2005 Dec;48(6):1070-82. doi: 10.1139/g05-062.