PMID- 16391700
OWN - NLM
STAT- MEDLINE
DCOM- 20060315
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 48
IP  - 5
DP  - 2005 Oct
TI  - 5S rDNA genome regions of Lens species.
PG  - 937-42
AB  - The length variability of the nontranscribed spacer (NTS) of the 5S rDNA repeats 
      was analyzed in species of the genus Lens by means of PCR amplification. The NTS 
      ranged from approximately 227 to approximately 952 bp. The polymorphism detected 
      was higher than previous NTS polymorphisms described in this genus. Three NTS 
      length variants from Lens culinaris subsp. culinaris and 2 from Lens culinaris 
      subsp. orientalis were sequenced. The culinaris NTS fragment lengths were 239, 
      371, and 838 bp, whereas the orientalis ones were 472 bp and 506 bp, 
      respectively. As a result of sequence similarities, 2 families of sequences were 
      distinguished, 1 including the sequences of 838 and 506 bp, and others with the 
      sequences of 239, 371, and 472 bp. The 1st family was characterized by the 
      presence of a repeated sequence designated A, whereas the 2nd family showed a 
      single A sequence and other repeated sequences designated B, C, and D. The 
      presence of an (AT)n microsatellite was also observed in the 2nd family of 
      sequences. The fragments, which included the 239-bp and 838-bp NTS sequences, as 
      well as the intergenic spacer (IGS) of the 18S-5.8S-26S ribosomal DNA also from 
      L. culinaris subsp. culinaris, were used to localize the nucleolar organizer 
      region (NOR) and the 5S rDNA loci in the chromosomes of several species of the 
      genus Lens by means of fluorescence in situ hybridization (FISH). The selective 
      hybridization of the 2 NTS probes allowed us to distinguish between different 5S 
      rDNA chromosomal loci.
FAU - Fernandez, M
AU  - Fernandez M
AD  - Area de Genetica, Facultad de Ciencias Biologicas y Ambientales, Universidad de 
      Leon, Spain.
FAU - Ruiz, M L
AU  - Ruiz ML
FAU - Linares, C
AU  - Linares C
FAU - Fominaya, A
AU  - Fominaya A
FAU - Perez de la Vega, M
AU  - Perez de la Vega M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Ribosomal)
RN  - 0 (DNA, Ribosomal Spacer)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - DNA, Ribosomal/genetics
MH  - DNA, Ribosomal Spacer/*genetics
MH  - Genome, Plant/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Lens Plant/*genetics
MH  - *Polymorphism, Genetic
MH  - RNA, Ribosomal, 5S/*genetics
EDAT- 2006/01/05 09:00
MHDA- 2006/03/16 09:00
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/03/16 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
AID - g05-052 [pii]
AID - 10.1139/g05-052 [doi]
PST - ppublish
SO  - Genome. 2005 Oct;48(5):937-42. doi: 10.1139/g05-052.