PMID- 16391789
OWN - NLM
STAT- MEDLINE
DCOM- 20070201
LR  - 20060104
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 28
IP  - 2
DP  - 2006 Feb
TI  - Intratumoral genetic heterogeneity in pilocytic astrocytomas revealed by 
      CGH-analysis of microdissected tumor cells and FISH on tumor tissue sections.
PG  - 353-60
AB  - Pilocytic astrocytomas are the most frequent gliomas of childhood. The majority 
      of cases show cytogenetic normal karyotypes. Although in diffuse gliomas TP53 
      gene mutations or deletions occur with significant frequency, the role in 
      pilocytic astrocytomas remains unclear. Histomorphologically different areas of 
      14 pilocytic astrocytomas were microdissected and analyzed for genetic 
      aberrations and heterogeneity. CGH analysis revealed gains of chromosome arm 4q 
      and 6q mainly in areas of classic biphasic pattern, whereas pleomorphic areas 
      presented gains of chromosome 6 and 8q. Using two-color fluorescence in situ 
      hybridization (FISH) the spatial distribution of aneuploidies for chromosomes 7, 
      8, 17 and the TP53 gene were assessed on parallel sections. FISH analysis 
      revealed a significant percentage of cells with interspersed heterozygous 
      deletions of TP53 in all tumors (14/14), ten cases showed also monosomy 17. 
      Besides gains of chromosomes 7 and 8, losses of these chromosomes were detected 
      in the majority of tumors. In conclusion, pilocytic astrocytomas show a genetic 
      heterogeneity associated with variations of histologic structure as well as an 
      intratumoral heterogeneity observed on single cell level by FISH.
FAU - Wemmert, Silke
AU  - Wemmert S
AD  - Department of Neurosurgery, Saarland University, D-66421 Homburg/Saar, Germany.
FAU - Romeike, Bernd F M
AU  - Romeike BF
FAU - Ketter, Ralf
AU  - Ketter R
FAU - Steudel, Wolf-Ingo
AU  - Steudel WI
FAU - Zang, Klaus D
AU  - Zang KD
FAU - Urbschat, Steffi
AU  - Urbschat S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Astrocytoma/*genetics/pathology
MH  - Central Nervous System Neoplasms/*genetics/pathology
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 7
MH  - Chromosomes, Human, Pair 8
MH  - Female
MH  - *Genes, p53
MH  - *Genetic Heterogeneity
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Male
MH  - Microdissection
MH  - Monosomy
MH  - Nucleic Acid Hybridization
EDAT- 2006/01/05 09:00
MHDA- 2007/02/03 09:00
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2007/02/03 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2006 Feb;28(2):353-60.