PMID- 16392133
OWN - NLM
STAT- MEDLINE
DCOM- 20060810
LR  - 20131121
IS  - 1549-3296 (Print)
IS  - 1549-3296 (Linking)
VI  - 77
IP  - 1
DP  - 2006 Apr
TI  - Chemokine gene activation in human bone marrow-derived osteoblasts following 
      exposure to particulate wear debris.
PG  - 192-201
AB  - Particulate wear debris induces the expression of pro-inflammatory cytokine and 
      chemokine genes in various cell types of the periprosthetic region. We have 
      previously reported that titanium particles stimulate the selective induction of 
      interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) chemokines in 
      human osteoblast-like osteosarcoma cells. In this study, we characterize the 
      human bone marrow-derived osteoblast chemokine response to titanium particles. We 
      demonstrate that titanium particles result in enhanced IL-8 and MCP-1 protein 
      secretion as well as differential chemokine gene activation. Osteoblast chemokine 
      expression was regulated at the level of gene transcription, with a 
      time-dependent induction of NF-kappaB activation. Inhibition studies with 
      N-acetyl-L-cysteine (Nac) and MG-132 suggest that titanium particle activation of 
      NF-kappaB activity and IL-8 chemokine expression involves oxidant signaling and 
      IkappaBalpha-proteasomal degradation. Activation of the NF-kappaB transcription 
      factor, as well as the IL-8 gene, are redox-regulated. We also demonstrate that 
      while cytochalasin D, a potent inhibitor of phagocytosis, suppressed the titanium 
      particle effect on IL-8 protein release in human bone marrow-derived osteoblasts, 
      the inhibitor had no effect on IL-8 expression in MG-63 osteoblast-like cells. 
      Collectively, these results provide insight into the potential mechanisms 
      responsible for the particulate activation of osteoblast chemokine expression and 
      suggest an important role for the osteoblast in the pathogenesis of 
      periprosthetic osteolysis.
CI  - (c) 2005 Wiley Periodicals, Inc.
FAU - Fritz, Elizabeth A
AU  - Fritz EA
AD  - Department of Immunology and Microbiology, Rush University Medical Center, 
      Chicago, Illinois 60612, USA.
FAU - Glant, Tibor T
AU  - Glant TT
FAU - Vermes, Csaba
AU  - Vermes C
FAU - Jacobs, Joshua J
AU  - Jacobs JJ
FAU - Roebuck, Kenneth A
AU  - Roebuck KA
LA  - eng
GR  - AR 45835/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 22144-77-0 (Cytochalasin D)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow Cells/cytology/*immunology/*physiology
MH  - Cell Line
MH  - Chemokine CCL2/immunology
MH  - Chemokines/*genetics/immunology
MH  - Cytochalasin D/metabolism
MH  - Female
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Interleukin-8/immunology
MH  - Male
MH  - NF-kappa B/antagonists & inhibitors/metabolism
MH  - Nucleic Acid Synthesis Inhibitors/metabolism
MH  - Particle Size
MH  - Phagocytosis/physiology
MH  - *Prostheses and Implants
MH  - Prosthesis Failure
MH  - Titanium/*immunology
MH  - Transcriptional Activation
EDAT- 2006/01/05 09:00
MHDA- 2006/08/11 09:00
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/08/11 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
AID - 10.1002/jbm.a.30609 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2006 Apr;77(1):192-201. doi: 10.1002/jbm.a.30609.