PMID- 16392139
OWN - NLM
STAT- MEDLINE
DCOM- 20060810
LR  - 20191210
IS  - 1549-3296 (Print)
IS  - 1549-3296 (Linking)
VI  - 77
IP  - 1
DP  - 2006 Apr
TI  - Controlled drug release from a novel injectable biodegradable 
      microsphere/scaffold composite based on poly(propylene fumarate).
PG  - 103-11
AB  - The ideal biomaterial for the repair of bone defects is expected to have good 
      mechanical properties, be fabricated easily into a desired shape, support cell 
      attachment, allow controlled release of bioactive factors to induce bone 
      formation, and biodegrade into nontoxic products to permit natural bone formation 
      and remodeling. The synthetic polymer poly(propylene fumarate) (PPF) holds great 
      promise as such a biomaterial. In previous work we developed 
      poly(DL-lactic-co-glycolic acid) (PLGA) and PPF microspheres for the controlled 
      delivery of bioactive molecules. This study presents an approach to incorporate 
      these microspheres into an injectable, porous PPF scaffold. Model drug Texas red 
      dextran (TRD) was encapsulated into biodegradable PLGA and PPF microspheres at 2 
      microg/mg microsphere. Five porous composite formulations were fabricated via a 
      gas foaming technique by combining the injectable PPF paste with the PLGA or PPF 
      microspheres at 100 or 250 mg microsphere per composite formulation, or a control 
      aqueous TRD solution (200 microg per composite). All scaffolds had an 
      interconnected pore network with an average porosity of 64.8 +/- 3.6%. The 
      presence of microspheres in the composite scaffolds was confirmed by scanning 
      electron microscopy and confocal microscopy. The composite scaffolds exhibited a 
      sustained release of the model drug for at least 28 days and had minimal burst 
      release during the initial phase of release, as compared to drug release from 
      microspheres alone. The compressive moduli of the scaffolds were between 2.4 and 
      26.2 MPa after fabrication, and between 14.9 and 62.8 MPa after 28 days in PBS. 
      The scaffolds containing PPF microspheres exhibited a significantly higher 
      initial compressive modulus than those containing PLGA microspheres. Increasing 
      the amount of microspheres in the composites was found to significantly decrease 
      the initial compressive modulus. The novel injectable PPF-based 
      microsphere/scaffold composites developed in this study are promising to serve as 
      vehicles for controlled drug delivery for bone tissue engineering.
CI  - (c) 2005 Wiley Periodicals, Inc.
FAU - Kempen, Diederik H R
AU  - Kempen DH
AD  - Tissue Engineering and Polymeric Biomaterials Laboratory, Department of 
      Orthopedic Surgery, Mayo Clinic College of Medicine, 200 First Street SW, 
      Rochester, Minnesota 55905, USA.
FAU - Lu, Lichun
AU  - Lu L
FAU - Kim, Choll
AU  - Kim C
FAU - Zhu, Xun
AU  - Zhu X
FAU - Dhert, Wouter J A
AU  - Dhert WJ
FAU - Currier, Bradford L
AU  - Currier BL
FAU - Yaszemski, Michael J
AU  - Yaszemski MJ
LA  - eng
GR  - R01 AR45871/AR/NIAMS NIH HHS/United States
GR  - R01 EB03060/EB/NIBIB NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Bone Cements)
RN  - 0 (Bone Substitutes)
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Dextrans)
RN  - 0 (Fumarates)
RN  - 0 (Polymers)
RN  - 0 (Polypropylenes)
RN  - 0 (Xanthenes)
RN  - 0 (poly(propylene fumarate))
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 82354-19-6 (Texas red)
SB  - IM
MH  - Bone Cements/chemistry/*metabolism
MH  - Bone Regeneration
MH  - *Bone Substitutes/chemistry/metabolism
MH  - Delayed-Action Preparations
MH  - Dextrans/metabolism
MH  - *Drug Delivery Systems
MH  - Fumarates/*metabolism
MH  - Injections
MH  - Lactic Acid/*metabolism
MH  - Materials Testing
MH  - Microscopy, Electron, Scanning
MH  - *Microspheres
MH  - Particle Size
MH  - Polyglycolic Acid/*metabolism
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*metabolism
MH  - Polypropylenes/*metabolism
MH  - Surface Properties
MH  - Tissue Engineering/methods
MH  - Xanthenes/metabolism
EDAT- 2006/01/05 09:00
MHDA- 2006/08/11 09:00
CRDT- 2006/01/05 09:00
PHST- 2006/01/05 09:00 [pubmed]
PHST- 2006/08/11 09:00 [medline]
PHST- 2006/01/05 09:00 [entrez]
AID - 10.1002/jbm.a.30336 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2006 Apr;77(1):103-11. doi: 10.1002/jbm.a.30336.