PMID- 16393513
OWN - NLM
STAT- MEDLINE
DCOM- 20060323
LR  - 20161020
IS  - 1008-1275 (Print)
IS  - 1008-1275 (Linking)
VI  - 9
IP  - 1
DP  - 2006 Feb
TI  - Establishment of a mechanical injury model of rat hippocampal neurons in vitro.
PG  - 29-33
AB  - OBJECTIVE: To establish a simple, reproducible, and practical mechanical injury 
      model of hippocampal neurons of Sprague-Dawley rats in vitro. METHODS: 
      Hippocampal neurons isolated from 1-2-day old rats were cultured in vitro. Mild, 
      moderate and severe mechanical injuries were delivered to the neurons by syringe 
      needle tearing, respectively. The control neurons were treated identically with 
      the exception of trauma. Cell damage was assessed by measuring the Propidium 
      Iodide (PI) uptaking at different time points (0.5, 1, 6, 12 and 24 hours) after 
      injury. The concentration of neuron specific enolase was also measured at some 
      time points. RESULTS: Pathological examination showed that degeneration, 
      degradation and necrosis occurred in the injured cultured neurons. Compared with 
      the control group, the ratio of PI-positive cells in the injured groups increased 
      significantly after 30 minutes of injury (P<0.05). More severe the damage was, 
      more PI-positive neurons were detected. Compared with the control group, the 
      concentration of neuron specific enolase in the injured culture increased 
      significantly after 1 hour of injury (P<0.05). CONCLUSIONS: The established model 
      of hippocampal neuron injury in vitro can be repeated easily and can simulate the 
      damage mechanism of traumatic brain injury, which can be used in the future 
      research of traumatic brain injury.
FAU - Yang, Xiao-feng
AU  - Yang XF
AD  - Department of Neurosurgery, Second Affiliated Hospital, Medical College of 
      Zhejiang University, Hangzhou 310009, China. yangxf@zju.edu.cn
FAU - Cao, Fei
AU  - Cao F
FAU - Pan, De-sheng
AU  - Pan DS
FAU - Liu, Wei-guo
AU  - Liu WG
FAU - Hu, Wei-wei
AU  - Hu WW
FAU - Zheng, Xiu-jue
AU  - Zheng XJ
FAU - Zhao, Xue-qun
AU  - Zhao XQ
FAU - Lu, Shi-ting
AU  - Lu ST
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin J Traumatol
JT  - Chinese journal of traumatology = Zhonghua chuang shang za zhi
JID - 100886162
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Brain Injuries/enzymology/*pathology
MH  - Equipment Design
MH  - Hippocampus/enzymology/*injuries
MH  - In Vitro Techniques
MH  - Neurons/enzymology/*pathology
MH  - Phosphopyruvate Hydratase/biosynthesis
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reproducibility of Results
EDAT- 2006/01/06 09:00
MHDA- 2006/03/24 09:00
CRDT- 2006/01/06 09:00
PHST- 2006/01/06 09:00 [pubmed]
PHST- 2006/03/24 09:00 [medline]
PHST- 2006/01/06 09:00 [entrez]
PST - ppublish
SO  - Chin J Traumatol. 2006 Feb;9(1):29-33.