PMID- 1639390
OWN - NLM
STAT- MEDLINE
DCOM- 19920828
LR  - 20190902
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 13
IP  - 3
DP  - 1992 Jul
TI  - Genomic organization, sequence analysis, and chromosomal localization of the 
      human carboxyl ester lipase (CEL) gene and a CEL-like (CELL) gene.
PG  - 630-40
AB  - The gene encoding human carboxyl ester lipase (CEL), including 1628 bp of the 
      5'-flanking region, has been isolated and characterized from two overlapping 
      lambda phage clones. The gene spans 9832 bp and contains 11 exons interrupted by 
      10 introns. The exons range in size from 88 to 204 bp, except for the last exon, 
      which is 841 bp. A major and a minor transcription initiation site were 
      determined 13 and 7 bp, respectively, upstream of the initiator methionine. The 
      nucleotide sequence is identical with that of the previously reported cDNA, 
      except for the third nucleotide in the 5'-untranslated sequence, a C, which in 
      the cDNA is a T. A TAAATA sequence is present 26 nt upstream from the major CAP 
      site, and within the 5'-flanking region there are several putative transcription 
      factor binding sites. Seven Alu repetitive sequence elements are present in the 
      region analyzed. The organization of the human CEL gene is similar to that of the 
      recently reported rat pancreatic cholesterol esterase gene. The CEL gene was 
      assigned to chromosome 9q34-qter, which confirms the recently reported results of 
      Tayler et al. (1991, Genomics 10: 425-431). A previously unknown gene with a 
      striking homology to the human CEL gene, here called the CEL-like gene (CELL), 
      has also been isolated and characterized, including 1724 bp of the 5'-flanking 
      region. The CELL gene, which most likely is a psuedogene, spans 4846 bp, and due 
      to the absence of a 4.8-kb segment, the CEL gene exons 2-7 are not present in the 
      CELL gene. Despite these differences, the CELL gene is transcribed. We have also 
      assigned the CELL gene to a separate locus at chromosome 9q34-qter.
FAU - Lidberg, U
AU  - Lidberg U
AD  - Department of Molecular Biology, University of Goteborg, Sweden.
FAU - Nilsson, J
AU  - Nilsson J
FAU - Stromberg, K
AU  - Stromberg K
FAU - Stenman, G
AU  - Stenman G
FAU - Sahlin, P
AU  - Sahlin P
FAU - Enerback, S
AU  - Enerback S
FAU - Bjursell, G
AU  - Bjursell G
LA  - eng
SI  - GENBANK/M87672
SI  - GENBANK/M87673
SI  - GENBANK/M87674
SI  - GENBANK/M87675
SI  - GENBANK/M87676
SI  - GENBANK/M87677
SI  - GENBANK/M87678
SI  - GENBANK/M87679
SI  - GENBANK/M94579
SI  - GENBANK/M94580
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.1.- (Carboxylic Ester Hydrolases)
RN  - EC 3.1.1.1 (Carboxylesterase)
SB  - IM
GS  - CEL
GS  - CELL
MH  - Animals
MH  - Base Sequence
MH  - Carboxylesterase
MH  - Carboxylic Ester Hydrolases/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 9
MH  - DNA/genetics
MH  - Exons
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Introns
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Rats
MH  - Sequence Homology, Nucleic Acid
MH  - Transcription, Genetic
EDAT- 1992/07/01 00:00
MHDA- 1992/07/01 00:01
CRDT- 1992/07/01 00:00
PHST- 1992/07/01 00:00 [pubmed]
PHST- 1992/07/01 00:01 [medline]
PHST- 1992/07/01 00:00 [entrez]
AID - 0888-7543(92)90134-E [pii]
AID - 10.1016/0888-7543(92)90134-e [doi]
PST - ppublish
SO  - Genomics. 1992 Jul;13(3):630-40. doi: 10.1016/0888-7543(92)90134-e.