PMID- 16396453
OWN - NLM
STAT- MEDLINE
DCOM- 20060324
LR  - 20240109
IS  - 0016-6758 (Print)
IS  - 0016-6758 (Linking)
VI  - 41
IP  - 12
DP  - 2005 Dec
TI  - [Investigation of chromosomes in varieties and translocation lines of pea Pisum 
      sativum L. by FISH, Ag-NOR, and differential DAPI staining].
PG  - 1665-73
AB  - The DNA intercalator 9-aminoachridine was used for obtaining high-resolution DAPI 
      patterns of chromosomes of Pisum sativum L. with more than 300 bands per haploid 
      chromosome set. The karyotypes of three pea varieties, Viola, Capital, and Rosa 
      Crown, and two translocation lines, L-108 (T(2-4s)) and M-10 (T(2-7s)), were 
      examined. Based on the results of DAPI staining, we have identified chromosomes, 
      constructed idiograms, and established breakpoints of chromosome translocations. 
      Lines L-108 (T(2-4s)) and M-10 (T(2-7s)) were shown to appear as a result of 
      respectively one translocation between chromosomes 2 and 4 and two translocations 
      between chromosomes 2 and 7. All varieties and translocation lines of pea were 
      examined using fluorescence in situ hybridization (FISH) with telomere repetition 
      probes, 5S and 45S wheat DNA probes. Transcriptional activity of 45S rRNA was 
      detected by Ag-NOR staining. Telomere repetitions were shown to be located only 
      in telomeric chromosome regions. Using high-resolution DAPI staining allowed us 
      to verify localization of 5S genes on pea chromosomes 1, 3, and 5. 45S rDNAs were 
      localized in the secondary constriction regions on the satellite and the 
      satellite thread of chromosome and on the satellite thread and in more proximal 
      satellite heterochromatic region of chromosome 7. The size of 45S rDNA signal on 
      chromosome 7 was larger and its transcriptional activity, higher than the 
      corresponding parameters on chromosome 4 in most of the forms studied. A visual 
      comparison of the results of FISH and Ag-NOR staining of normal and translocated 
      pea chromosomes did not reveal any significant differences between them. The 
      translocations of the satellite chromosomes apparently did not cause significant 
      changes either in the amount of the ribosomal genes or in their transcriptional 
      activity.
FAU - Samatadze, T E
AU  - Samatadze TE
FAU - Muravenko, O M
AU  - Muravenko OM
FAU - Bol'sheva, N L
AU  - Bol'sheva NL
FAU - Amosova, A B
AU  - Amosova AB
FAU - Gostimsckii, S A
AU  - Gostimsckii SA
FAU - Zelenin, A V
AU  - Zelenin AV
LA  - rus
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Genetika
JT  - Genetika
JID - 0047354
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Chromosome Banding
MH  - Chromosomes, Plant/*genetics
MH  - DNA Probes
MH  - DNA, Ribosomal/*genetics
MH  - DNA, Satellite/*genetics
MH  - Haploidy
MH  - In Situ Hybridization/methods
MH  - Pisum sativum/*genetics
MH  - Translocation, Genetic/*genetics
EDAT- 2006/01/07 09:00
MHDA- 2006/03/25 09:00
CRDT- 2006/01/07 09:00
PHST- 2006/01/07 09:00 [pubmed]
PHST- 2006/03/25 09:00 [medline]
PHST- 2006/01/07 09:00 [entrez]
PST - ppublish
SO  - Genetika. 2005 Dec;41(12):1665-73.