PMID- 16396761
OWN - NLM
STAT- MEDLINE
DCOM- 20060803
LR  - 20190116
IS  - 1042-8194 (Print)
IS  - 1026-8022 (Linking)
VI  - 47
IP  - 3
DP  - 2006 Mar
TI  - Fluorescent in situ hybridization in the diagnosis, prognosis, and treatment 
      monitoring of chronic myeloid leukemia.
PG  - 397-402
AB  - The unique molecular characteristic of chronic myeloid leukemia (CML), the 
      disease-causing ABL (9q34) to BCR (22q11) translocation, has provided an 
      invaluable tool for disease diagnosis and monitoring of treatment response. The 
      traditional standard in this regard is bone marrow karyotype, also known as 
      conventional cytogenetics (CC), which reveals a shortened chromosome 22, the 
      Philadelphia chromosome, t(9;22)(q34;q11). CC in CML has also been effectively 
      used for monitoring the response to drug therapy. However, this particular 
      laboratory test misses submicroscopic BCR/ABL translocations and is suboptimal 
      for minimal residual disease (MRD) assessment. Both fluorescence in situ 
      hybridization (FISH) and reverse-transcriptase polymerase chain reaction (RT-PCR) 
      feature higher sensitivity in terms of both diagnosis and MRD assessment in CML, 
      compared to CC. Another advantage of these alternative tests is their effective 
      applicability to peripheral blood specimens. The current review highlights the 
      practical literature with respect to the use of FISH for CML whereas the use of 
      RT-PCR has been extensively covered in recent communications.
FAU - Landstrom, Andrew P
AU  - Landstrom AP
AD  - Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
FAU - Tefferi, Ayalew
AU  - Tefferi A
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/*drug 
      therapy/pathology
MH  - Prognosis
MH  - Reverse Transcriptase Polymerase Chain Reaction
RF  - 65
EDAT- 2006/01/07 09:00
MHDA- 2006/08/04 09:00
CRDT- 2006/01/07 09:00
PHST- 2006/01/07 09:00 [pubmed]
PHST- 2006/08/04 09:00 [medline]
PHST- 2006/01/07 09:00 [entrez]
AID - G833138XV761U316 [pii]
AID - 10.1080/10428190500353133 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2006 Mar;47(3):397-402. doi: 10.1080/10428190500353133.