PMID- 16397773
OWN - NLM
STAT- MEDLINE
DCOM- 20071026
LR  - 20181113
IS  - 0300-5623 (Print)
IS  - 0300-5623 (Linking)
VI  - 34
IP  - 1
DP  - 2006 Feb
TI  - Reactive oxygen species mediated calcium oxalate crystal-induced expression of 
      MCP-1 in HK-2 cells.
PG  - 26-36
AB  - Under severe hyperoxaluric conditions calcium oxalate crystals often deposit in 
      the renal interstitium and produce localized inflammation. We have proposed that 
      renal epithelial cells exposed to CaOx crystals produce chemoattractants such as 
      monocyte chemoattractant protein-1 (MCP-1). MCP-1 synthesis is mediated by 
      reactive oxygen species (ROS). HK-2 cells of human renal epithelial line were 
      exposed to CaOx crystals for different lengths of time. The culture media was 
      tested for cell injury marker LDH, and subjected to enzyme-linked immunosorbent 
      assay to determine the secretion of MCP-1 protein. Cell expression of MCP-1 was 
      assessed by Western blot analysis. Gene expression was determined by reverse 
      transcriptase-polymerase chain reaction. The data clearly showed that the HK-2 
      cells express MCP-1 gene and protein. The MCP-1 mRNA expression was increased 
      following exposure to CaOx crystals, which was reduced upon treatment with free 
      radical scavengers, catalase and superoxide dismutase. Results indicate that CaOx 
      crystals strongly induce MCP-1 synthesis and secretion by the HK-2 cells and 
      production is mediated by intracellular ROS production. Based on these and other 
      data, antioxidant therapy and blockade of rennin-angiotensin system may prove 
      beneficial for the prevention of end stage renal disease caused by hyperoxaluria 
      and CaOx crystal deposition.
FAU - Habibzadegah-Tari, Pouran
AU  - Habibzadegah-Tari P
AD  - Department of Pathology and Laboratory Medicine, University of Florida College of 
      Medicine, 100275, Gainesville, FL 32610-0275, USA.
FAU - Byer, Karen G
AU  - Byer KG
FAU - Khan, Saeed R
AU  - Khan SR
LA  - eng
GR  - DK053962/DK/NIDDK NIH HHS/United States
GR  - DK059765/DK/NIDDK NIH HHS/United States
GR  - DK065658/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060106
PL  - Germany
TA  - Urol Res
JT  - Urological research
JID - 0364311
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 11062-77-4 (Superoxides)
RN  - 2612HC57YE (Calcium Oxalate)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Blotting, Western
MH  - Calcium Oxalate/*pharmacology
MH  - Cell Line
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/cytology/metabolism
MH  - Free Radical Scavengers/metabolism
MH  - Gene Expression/physiology
MH  - Humans
MH  - Kidney/cytology
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Lipid Peroxidation/drug effects/physiology
MH  - Nephritis, Interstitial/immunology/*metabolism
MH  - Oxidative Stress/*drug effects/physiology
MH  - RNA, Messenger/metabolism
MH  - Reactive Oxygen Species/*metabolism
MH  - Superoxides/metabolism
EDAT- 2006/01/07 09:00
MHDA- 2007/10/30 09:00
CRDT- 2006/01/07 09:00
PHST- 2005/10/18 00:00 [received]
PHST- 2005/12/05 00:00 [accepted]
PHST- 2006/01/07 09:00 [pubmed]
PHST- 2007/10/30 09:00 [medline]
PHST- 2006/01/07 09:00 [entrez]
AID - 10.1007/s00240-005-0007-3 [doi]
PST - ppublish
SO  - Urol Res. 2006 Feb;34(1):26-36. doi: 10.1007/s00240-005-0007-3. Epub 2006 Jan 6.