PMID- 16397795
OWN - NLM
STAT- MEDLINE
DCOM- 20060531
LR  - 20181203
IS  - 0948-6143 (Print)
IS  - 0948-6143 (Linking)
VI  - 125
IP  - 1-2
DP  - 2006 Jan
TI  - Role of H1 phosphorylation in rapid GR exchange and function at the MMTV 
      promoter.
PG  - 83-9
AB  - Photobleaching technology has demonstrated in live cells that the glucocorticoid 
      receptor (GR) exchanges rapidly at the mouse mammary tumor virus (MMTV) promoter. 
      GR rapid exchange at MMTV depends on chaperone and proteasome activity, and as 
      suggested by several in vitro and in vivo biochemical approaches, may also 
      involve chromatin remodeling activity. Inhibition of H1 phosphorylation, 
      chromatin remodeling and transcription from MMTV can be accomplished by long-term 
      blocking of Cdk2 protein kinase activity. We find that Cdk2 is recruited by a 
      tandem array of MMTV promoters, strengthening the model that this kinase has a 
      specific role in MMTV transcription. We also demonstrate that following a brief 
      Cdk2 inhibition by a selective cyclin-dependent kinase inhibitor (Roscovitine), 
      transcription from MMTV drops and GR exchange at MMTV becomes slower, with a 
      fraction of GR molecules now tightly bound at the promoter. This immobile 
      fraction is absent elsewhere in the nucleus, suggesting a specific effect of Cdk2 
      inhibition on GR-MMTV interactions. These are the first live cell data suggesting 
      a role for H1 phosphorylation, and by implication chromatin remodeling, in rapid 
      exchange of GR at MMTV.
FAU - Stavreva, Diana A
AU  - Stavreva DA
AD  - Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, 
      National Cancer Institute, NIH, Bldg 41, Room B516, Bethesda, MD 20892, USA.
FAU - McNally, James G
AU  - McNally JG
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20051117
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Histones)
RN  - 0 (Purines)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0ES1C2KQ94 (Roscovitine)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 2)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cyclin-Dependent Kinase 2/genetics
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/pharmacology
MH  - Histones/*metabolism/physiology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Phosphorylation
MH  - Promoter Regions, Genetic/*genetics
MH  - Purines/pharmacology
MH  - RNA/biosynthesis/genetics
MH  - Receptors, Glucocorticoid/*metabolism
MH  - Roscovitine
MH  - Transcription, Genetic/drug effects
EDAT- 2006/01/07 09:00
MHDA- 2006/06/01 09:00
CRDT- 2006/01/07 09:00
PHST- 2005/09/14 00:00 [accepted]
PHST- 2006/01/07 09:00 [pubmed]
PHST- 2006/06/01 09:00 [medline]
PHST- 2006/01/07 09:00 [entrez]
AID - 10.1007/s00418-005-0086-9 [doi]
PST - ppublish
SO  - Histochem Cell Biol. 2006 Jan;125(1-2):83-9. doi: 10.1007/s00418-005-0086-9. Epub 
      2005 Nov 17.