PMID- 16398432
OWN - NLM
STAT- MEDLINE
DCOM- 20071220
LR  - 20161018
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 25
IP  - 12
DP  - 2005 Dec
TI  - [Experimental study on replicating knee osteoarthritis by modified Hulth's 
      modeling method].
PG  - 1104-8
AB  - OBJECTIVE: To explore the method of replicating experimental animal model of knee 
      osteoarthritis. METHODS: Knee osteoarthritis was replicated by modified Hulth's 
      modeling method. X-ray photographic and transmission electron microscopic 
      examination, test of the joint synovial fluid of the modeled joint were 
      performed, and serum contents of malondialdehyde (MDA) and nitric oxide (NO) in 
      blood were measured. RESULTS: (1) In the normal control group, the articular 
      surface is smooth and glossy, with intact cells and cellular membrane. In the 
      model group, the medial space of the knee joint became obvious narrowed with 
      rough and deformed articular surface and osteophytes, as well as the atrophic 
      chondrocytes with pyknotic cell nucleus and broken cellular membrane. (2) Eight 
      weeks and 16 weeks after modeling, in the model group, the contents of 
      interleukin-1 (IL-1), interleukin-6 (IL-6), tumor necrosis factor-alpha 
      (TNF-alpha) and matrix metalloproteinase-3 (MMP-3) in synovial fluid and the 
      levels of serum MDA and NO were obviously raised, and the activity of superoxide 
      dismutase (SOD) in synovial membrane was obviously lowered 16 weeks after 
      modeling, showing significant difference when compared with those in the normal 
      control group (P < 0.01). CONCLUSION: Modified Hulth's modeling method in 
      replicating knee osteoarthritis is simple in manipulation with less wound, and 
      the condition of modeled knee joint could be maintained stable to certain degree, 
      which is advantageous to the success of animal model replicating.
FAU - Liu, Xian-xiang
AU  - Liu XX
AD  - Academy of Intergrative Medicine, Fujian College of Traditional Chinese Medicine, 
      Fuzhou 350000. liuxianxiang@163.com
FAU - Li, Xi-hai
AU  - Li XH
FAU - Zhou, Jiang-tao
AU  - Zhou JT
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
SB  - IM
MH  - Animals
MH  - *Disease Models, Animal
MH  - Female
MH  - Male
MH  - *Osteoarthritis, Knee
MH  - Rabbits
EDAT- 2006/01/10 09:00
MHDA- 2007/12/21 09:00
CRDT- 2006/01/10 09:00
PHST- 2006/01/10 09:00 [pubmed]
PHST- 2007/12/21 09:00 [medline]
PHST- 2006/01/10 09:00 [entrez]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2005 Dec;25(12):1104-8.