PMID- 16398459
OWN - NLM
STAT- MEDLINE
DCOM- 20060712
LR  - 20191026
IS  - 1092-0684 (Electronic)
IS  - 1092-0684 (Linking)
VI  - 19
IP  - 5
DP  - 2005 Nov 15
TI  - Molecular cytogenetic analysis in the study of brain tumors: findings and 
      applications.
PG  - E1
AB  - Classic cytogenetics has evolved from black and white to technicolor images of 
      chromosomes as a result of advances in fluorescence in situ hybridization (FISH) 
      techniques, and is now called molecular cytogenetics. Improvements in the quality 
      and diversity of probes suitable for FISH, coupled with advances in computerized 
      image analysis, now permit the genome or tissue of interest to be analyzed in 
      detail on a glass slide. It is evident that the growing list of options for 
      cytogenetic analysis has improved the understanding of chromosomal changes in 
      disease initiation, progression, and response to treatment. The contributions of 
      classic and molecular cytogenetics to the study of brain tumors have provided 
      scientists and clinicians alike with new avenues for investigation. In this 
      review the authors summarize the contributions of molecular cytogenetics to the 
      study of brain tumors, encompassing the findings of classic cytogenetics, 
      interphase- and metaphase-based FISH studies, spectral karyotyping, and 
      metaphase- and array-based comparative genomic hybridization. In addition, this 
      review also details the role of molecular cytogenetic techniques in other aspects 
      of understanding the pathogenesis of brain tumors, including xenograft, cancer 
      stem cell, and telomere length studies.
FAU - Bayani, Jane
AU  - Bayani J
AD  - Department of Applied Molecular Oncology, Ontario Cancer Institute, Princess 
      Margaret Hospital, University Health Network, Ontario, Canada. 
      jane.bayani@utoronto.ca
FAU - Pandita, Ajay
AU  - Pandita A
FAU - Squire, Jeremy A
AU  - Squire JA
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20051115
PL  - United States
TA  - Neurosurg Focus
JT  - Neurosurgical focus
JID - 100896471
SB  - IM
MH  - Animals
MH  - Brain Neoplasms/diagnosis/*genetics
MH  - Cytogenetic Analysis/*methods/trends
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods/trends
MH  - Karyotyping/methods
RF  - 307
EDAT- 2006/01/10 09:00
MHDA- 2006/07/13 09:00
CRDT- 2006/01/10 09:00
PHST- 2006/01/10 09:00 [pubmed]
PHST- 2006/07/13 09:00 [medline]
PHST- 2006/01/10 09:00 [entrez]
AID - 190501 [pii]
AID - 10.3171/foc.2005.19.5.2 [doi]
PST - epublish
SO  - Neurosurg Focus. 2005 Nov 15;19(5):E1. doi: 10.3171/foc.2005.19.5.2.