PMID- 1639916
OWN - NLM
STAT- MEDLINE
DCOM- 19920828
LR  - 20190629
VI  - 593
IP  - 1-2
DP  - 1992 Feb 28
TI  - Determination of psychotropic phenylalkylamine derivatives in biological matrices 
      by high-performance liquid chromatography with photodiode-array detection.
PG  - 87-94
AB  - Several procedures using high-performance liquid chromatography with 
      photodiode-array detection have been developed to create phytochemical and 
      toxicological profiles of phenylalkylamine derivatives in biological samples 
      (e.g. plant materials and urine). Mescaline-containing cactus samples were 
      extracted with basic methanol, using methoxamine as internal standard; the 
      extraction and clean-up of urine samples were performed on cation-exchange 
      solid-phase extraction columns. The extracts were separated on a 3-micron ODS 
      column with acetonitrile-water-phosphoric acid-hexylamine as the mobile phase. 
      Peak detection was performed at 198 or 205 nm; peak identity and homogeneity were 
      ascertained by on-line scanning of the UV spectra from 190 to 300 nm. The 
      detection limit of phenylalkylamine derivatives in urine and cactus material was 
      0.026-0.056 micrograms/ml and 0.04 micrograms/mg, respectively. Following a 
      single oral dose of 1.7 mg/kg methylenedioxymethylamphetamine (MDMA) the 
      concentrations found in urine ranged from 1.48 to 5.05 micrograms/ml MDMA and 
      0.07-0.90 micrograms/ml methylenedioxyamphetamine (a metabolite of MDMA). The 
      mescaline content of the cactus Trichocereus pachanoi varied between 1.09 and 
      23.75 micrograms/mg.
FAU - Helmlin, H J
AU  - Helmlin HJ
AD  - Institute of Pharmacy, University of Berne, Switzerland.
FAU - Brenneisen, R
AU  - Brenneisen R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr
JT  - Journal of chromatography
JID - 0427043
RN  - 0 (Amphetamines)
RN  - 0 (Designer Drugs)
RN  - C045TQL4WP (Phentermine)
RN  - HUQ1KC1YLI (Methoxamine)
RN  - RHO99102VC (Mescaline)
SB  - IM
MH  - Amphetamines/*urine
MH  - Chromatography, High Pressure Liquid
MH  - *Designer Drugs
MH  - Humans
MH  - Mescaline/*urine
MH  - Methoxamine/*urine
MH  - Phentermine/*urine
MH  - Plants/*chemistry
MH  - Reproducibility of Results
MH  - Spectrophotometry, Ultraviolet
EDAT- 1992/02/28 00:00
MHDA- 1992/02/28 00:01
CRDT- 1992/02/28 00:00
PHST- 1992/02/28 00:00 [pubmed]
PHST- 1992/02/28 00:01 [medline]
PHST- 1992/02/28 00:00 [entrez]
AID - 10.1016/0021-9673(92)80271-u [doi]
PST - ppublish
SO  - J Chromatogr. 1992 Feb 28;593(1-2):87-94. doi: 10.1016/0021-9673(92)80271-u.