PMID- 16399788
OWN - NLM
STAT- MEDLINE
DCOM- 20060616
LR  - 20200930
IS  - 1040-0605 (Print)
IS  - 1040-0605 (Linking)
VI  - 290
IP  - 5
DP  - 2006 May
TI  - Moraxella catarrhalis induces inflammatory response of bronchial epithelial cells 
      via MAPK and NF-kappaB activation and histone deacetylase activity reduction.
PG  - L818-26
AB  - Moraxella catarrhalis is a major cause of infectious exacerbations of chronic 
      obstructive lung disease (COPD) and may also contribute to the pathogenesis of 
      COPD. Little is known about M. catarrhalis-bronchial epithelium interaction. We 
      investigated activation of M. catarrhalis infected bronchial epithelial cells and 
      characterized the signal transduction pathways. Moreover, we tested the 
      hypothesis that the M. catarrhalis-induced cytokine expression is regulated by 
      acetylation of histone residues and controlled by histone deacetylase activity 
      (HDAC). We demonstrated that M. catarrhalis induced a strong time- and 
      dose-dependent inflammatory response in the bronchial epithelial cell line 
      (BEAS-2B), characterized by the release of IL-8 and GM-CSF. For this cytokine 
      liberation activation of the ERK and p38 mitogen-activated protein (MAP) kinases 
      and transcription factor NF-kappaB was required. Furthermore, M. 
      catarrhalis-infected bronchial epithelial cells showed an enhanced acetylation of 
      histone H3 and H4 globally and at the promoter of the il8 gene. Preventing 
      histone deacetylation by the histone deacetylase inhibitor trichostatin A 
      augmented the M. catarrhalis-induced IL-8 response. After exposure to M. 
      catarrhalis, we found a decrease in global histone deacetylase expression and 
      activity. Our findings suggest that M. catarrhalis-induced activation of il8 gene 
      transcription was caused by interference with epigenetic mechanisms regulating 
      il8 gene accessibility. Our findings provide insight into important molecular and 
      cellular mechanisms of M. catarrhalis-induced activation of human bronchial 
      epithelium.
FAU - Slevogt, Hortense
AU  - Slevogt H
AD  - Dept. of Internal Medicine/Infectious Diseases, Charite-Universitatsmedizin 
      Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. 
      hortense.slevogt@charite.de
FAU - Schmeck, Bernd
AU  - Schmeck B
FAU - Jonatat, Carola
AU  - Jonatat C
FAU - Zahlten, Janine
AU  - Zahlten J
FAU - Beermann, Wiebke
AU  - Beermann W
FAU - van Laak, Vincent
AU  - van Laak V
FAU - Opitz, Bastian
AU  - Opitz B
FAU - Dietel, Solveig
AU  - Dietel S
FAU - N'Guessan, Philippe Dje
AU  - N'Guessan PD
FAU - Hippenstiel, Stefan
AU  - Hippenstiel S
FAU - Suttorp, Norbert
AU  - Suttorp N
FAU - Seybold, Joachim
AU  - Seybold J
LA  - eng
PT  - Journal Article
DEP - 20060106
PL  - United States
TA  - Am J Physiol Lung Cell Mol Physiol
JT  - American journal of physiology. Lung cellular and molecular physiology
JID - 100901229
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Histone Deacetylase Inhibitors)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - Bronchi/*microbiology/*physiopathology
MH  - Cell Line
MH  - Enzyme Inhibitors/pharmacology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/physiology
MH  - Histone Deacetylase Inhibitors
MH  - Histone Deacetylases/*metabolism
MH  - Humans
MH  - Inflammation/*microbiology
MH  - Interleukin-8/physiology
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Moraxella catarrhalis/*physiology
MH  - NF-kappa B/*metabolism
MH  - Respiratory Mucosa/*physiopathology
EDAT- 2006/01/10 09:00
MHDA- 2006/06/17 09:00
CRDT- 2006/01/10 09:00
PHST- 2006/01/10 09:00 [pubmed]
PHST- 2006/06/17 09:00 [medline]
PHST- 2006/01/10 09:00 [entrez]
AID - 00428.2005 [pii]
AID - 10.1152/ajplung.00428.2005 [doi]
PST - ppublish
SO  - Am J Physiol Lung Cell Mol Physiol. 2006 May;290(5):L818-26. doi: 
      10.1152/ajplung.00428.2005. Epub 2006 Jan 6.