PMID- 16400001
OWN - NLM
STAT- MEDLINE
DCOM- 20060425
LR  - 20061115
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 54
IP  - 4
DP  - 2006 Apr
TI  - Expression of telomerase reverse transcriptase subunit (TERT) and telomere sizing 
      in pig ovarian follicles.
PG  - 443-55
AB  - Telomerase is crucial for chromosome stability because it maintains telomere 
      length. Little is known about telomerase in ovarian follicles, where an intense 
      cell division is crucial to sustain estrous cycle and to drive oocyte 
      development. The present research was performed to detect, by 
      immunohistochemistry, the distribution of telomerase catalytic subunit (TERT) 
      during folliculogenesis and to study the effect of TERT expression on telomeres. 
      To this aim, telomere length has been measured on fluorescence in situ 
      hybridization (FISH)-processed sections either in follicular or in germ cells. In 
      primary and preantral follicles, TERT was observed in granulosa and in germ 
      cells, with a typical nuclear location. During antral differentiation, only 
      somatic cells close to the antrum (antral layer) and cumulus cells maintained 
      TERT expression. The relative oocytes located TERT in the ooplasm independent 
      from the process of meiotic maturation. FISH results indicate that a correlation 
      exists between TERT expression and telomere size. In fact, progressively bigger 
      telomeres were observed from preantral to antral follicles where longer 
      structures were recorded in cells of the cumulus oophorus and of the antral layer 
      than those of the basal one. Stable and elongated telomeres were detected in 
      fully grown oocytes that lost the functional TERT distribution within the 
      nucleus.
FAU - Russo, Valentina
AU  - Russo V
AD  - Dipartimento di Scienze Biomediche Comparate, Universita degli Studi di Teramo, 
      64100 Teramo, Italy. vrusso@unite.it
FAU - Berardinelli, Paolo
AU  - Berardinelli P
FAU - Martelli, Alessandra
AU  - Martelli A
FAU - Di Giacinto, Oriana
AU  - Di Giacinto O
FAU - Nardinocchi, Delia
AU  - Nardinocchi D
FAU - Fantasia, Donatella
AU  - Fantasia D
FAU - Barboni, Barbara
AU  - Barboni B
LA  - eng
PT  - Journal Article
DEP - 20060106
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Protein Subunits)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Animals
MH  - Cell Compartmentation
MH  - DNA-Binding Proteins/*biosynthesis
MH  - Female
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Oocytes/metabolism/ultrastructure
MH  - Ovarian Follicle/*enzymology/ultrastructure
MH  - Protein Subunits/metabolism
MH  - Swine
MH  - Telomerase/*biosynthesis
MH  - Telomere/*ultrastructure
EDAT- 2006/01/10 09:00
MHDA- 2006/04/28 09:00
CRDT- 2006/01/10 09:00
PHST- 2006/01/10 09:00 [pubmed]
PHST- 2006/04/28 09:00 [medline]
PHST- 2006/01/10 09:00 [entrez]
AID - jhc.4A6603.2006 [pii]
AID - 10.1369/jhc.4A6603.2006 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2006 Apr;54(4):443-55. doi: 10.1369/jhc.4A6603.2006. Epub 
      2006 Jan 6.