PMID- 16400084
OWN - NLM
STAT- MEDLINE
DCOM- 20060424
LR  - 20061115
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 27
IP  - 1
DP  - 2006 Jan-Feb
TI  - Studies on varicocele III: ultrastructural sperm evaluation and 18, X and Y 
      aneuploidies.
PG  - 94-101
AB  - The idea that varicocele plays a detrimental role in fertility is supported by 
      the presence of a higher frequency of affected men among the infertile population 
      than among men with normal semen parameters. In this research we examined 
      ejaculates from a large group of selected men affected by varicocele by light and 
      electron microscopy. The effect of varicocele on chromosome meiotic segregation 
      was investigated by fluorescence in situ hybridization (FISH). The potential 
      benefits of varicocelectomy on sperm quality were evaluated by analyzing sperm 
      characteristics before and after surgical correction of varicocele. Transmission 
      electron microscopy (TEM) analysis, elaborated previously, showed that the 
      incidence of immaturity, apoptosis, and necrosis was higher in the varicocele 
      group than in controls. FISH analysis performed on sperm nuclei from selected 
      patients with varicocele showed that the mean frequencies of disomies and 
      diploidies were generally out of the normal range, indicating a severe 
      disturbance in meiotic segregation. Sperm characteristics evaluated before and 
      after varicocele repair showed a general improvement. As a consequence, the 
      varicocele seem to affect sperm morphology and function concomitantly with 
      meiotic segregation derangement. In consideration of these data, we suggest that 
      TEM and FISH analyses should be performed for all varicocele patients.
FAU - Baccetti, Baccio M
AU  - Baccetti BM
AD  - Department of Pediatrics, Obstetrics and Reproductive Medicine, Biology Section, 
      University of Siena, Policlinico S. Maria alle Scotte, 53100 Siena, Italy.
FAU - Bruni, Emanuele
AU  - Bruni E
FAU - Capitani, Serena
AU  - Capitani S
FAU - Collodel, Giulia
AU  - Collodel G
FAU - Mancini, Stefano
AU  - Mancini S
FAU - Piomboni, Paola
AU  - Piomboni P
FAU - Moretti, Elena
AU  - Moretti E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Case-Control Studies
MH  - *Chromosomes, Human, Pair 18
MH  - *Chromosomes, Human, X
MH  - *Chromosomes, Human, Y
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Polymerase Chain Reaction
MH  - Spermatozoa/*ultrastructure
MH  - Varicocele/*genetics/surgery
EDAT- 2006/01/10 09:00
MHDA- 2006/04/25 09:00
CRDT- 2006/01/10 09:00
PHST- 2006/01/10 09:00 [pubmed]
PHST- 2006/04/25 09:00 [medline]
PHST- 2006/01/10 09:00 [entrez]
AID - 27/1/94 [pii]
AID - 10.2164/jandrol.05081 [doi]
PST - ppublish
SO  - J Androl. 2006 Jan-Feb;27(1):94-101. doi: 10.2164/jandrol.05081.