PMID- 16400604
OWN - NLM
STAT- MEDLINE
DCOM- 20060525
LR  - 20181113
IS  - 0165-7380 (Print)
IS  - 0165-7380 (Linking)
VI  - 30
IP  - 2
DP  - 2006 Feb
TI  - Detection of foot and mouth disease and porcine reproductive and respiratory 
      syndrome viral genes using microarray chip.
PG  - 191-204
AB  - Two viral pathogens, namely, porcine reproductive and respiratory syndrome virus 
      (PRRSV) and foot and mouth disease virus (FMDV), were selected as models for 
      multiple pathogen detection in a cDNA microarray. Two signature regions selected 
      from ORF2 (around 500 bp) and ORF5 (around 600 bp) of PRRVS (America serotype), 
      and one signature region from structural genes VP1 (around 500 bp) of FMDV type O 
      were designed and spotted on a nylon membrane. For PCR sensitivity study, the 
      cloned FMDV-VP1 template could be diluted to near one copy and its PCR product 
      was still detectable in gel electrophoresis. In the microarray detection, the 
      labelling FMDV probes (3 mg/ml) could be diluted 320 times and still maintained a 
      visible colour when hybridized with the chip. Using the mixing primers, the 
      microarray chip demonstrated rapid and accurate detection of the specific genes. 
      To our knowledge, this preliminary study is the first example reported applying 
      the long signature sequences to the multiple pathogen detection in cDNA 
      microarray.
FAU - Liu, Y-C
AU  - Liu YC
AD  - Department of Chemical Engineering, National Chung Hsing University, Taichung 
      402, Taiwan. ycliu@dragon.nchu.edu.tw
FAU - Huang, G S
AU  - Huang GS
FAU - Wu, M-C
AU  - Wu MC
FAU - Hong, M-Y
AU  - Hong MY
FAU - Hsiung, K-P
AU  - Hsiung KP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Switzerland
TA  - Vet Res Commun
JT  - Veterinary research communications
JID - 8100520
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Animals
MH  - Foot-and-Mouth Disease Virus/*genetics/isolation & purification
MH  - Image Processing, Computer-Assisted
MH  - Oligonucleotide Array Sequence Analysis/*veterinary
MH  - Open Reading Frames/genetics
MH  - Pilot Projects
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification
MH  - RNA, Viral/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/diagnosis/*virology
MH  - Viral Structural Proteins/genetics
EDAT- 2006/01/10 09:00
MHDA- 2006/05/26 09:00
CRDT- 2006/01/10 09:00
PHST- 2004/09/21 00:00 [accepted]
PHST- 2006/01/10 09:00 [pubmed]
PHST- 2006/05/26 09:00 [medline]
PHST- 2006/01/10 09:00 [entrez]
AID - 10.1007/s11259-006-3193-8 [doi]
PST - ppublish
SO  - Vet Res Commun. 2006 Feb;30(2):191-204. doi: 10.1007/s11259-006-3193-8.