PMID- 16400995
OWN - NLM
STAT- MEDLINE
DCOM- 20060208
LR  - 20061115
IS  - 0026-3656 (Print)
IS  - 0026-3656 (Linking)
VI  - 74
IP  - 6
DP  - 2005 Nov-Dec
TI  - [Estimation of the phylogenetic diversity of prokaryotic microorganisms in 
      Sphagnum bogs with the use of fluorescence in situ hybridization (FISH)].
PG  - 831-7
AB  - The microbial population of Sphagnum bogs of northern Russia was analyzed with 
      respect to the presence and cell numbers of representatives of particular 
      phylogenetic groups of prokaryotes by means of in situ hybridization with 
      fluorescently labeled rRNA-targeted oligonucleotide probes with broad detection 
      spectra. The total number of cells that hybridized with universal Archaea- or 
      Bacteria-specific probes varied, in peat samples of different bogs, from 45 to 
      83% of the number of cells revealed by DAPI staining. Down the bog profiles, the 
      total number of prokaryotes and the fraction of archaea among them increased. 
      Application of a set of oligonucleotide probes showed that the number of 
      microorganisms belonging to such phylogenetic lineages of the domain Bacteria as 
      the phyla Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, 
      Acidobacteria, and Planctomycetes constituted, in total, 14.0-26.5% of the number 
      of eubacteria detected in the samples. Among the bacteria identified in the peat 
      samples, the most abundant were representatives of the classes 
      Alphaproteobacteria and Betaproteobacteria and the phyla Acidobacteria, 
      Bacteroidetes, and Actinobacteria.
FAU - Pankratov, T A
AU  - Pankratov TA
FAU - Belova, S E
AU  - Belova SE
FAU - Dedysh, S N
AU  - Dedysh SN
LA  - rus
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Mikrobiologiia
JT  - Mikrobiologiia
JID - 0376652
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - Archaea/genetics/*isolation & purification
MH  - Bacteria/genetics/*isolation & purification
MH  - In Situ Hybridization, Fluorescence
MH  - Oligonucleotide Probes
MH  - RNA, Ribosomal/genetics
MH  - Russia
MH  - *Soil Microbiology
EDAT- 2006/01/13 09:00
MHDA- 2006/02/09 09:00
CRDT- 2006/01/13 09:00
PHST- 2006/01/13 09:00 [pubmed]
PHST- 2006/02/09 09:00 [medline]
PHST- 2006/01/13 09:00 [entrez]
PST - ppublish
SO  - Mikrobiologiia. 2005 Nov-Dec;74(6):831-7.