PMID- 16402030
OWN - NLM
STAT- MEDLINE
DCOM- 20060518
LR  - 20230216
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 86
IP  - 3
DP  - 2006 Mar
TI  - Effects of bile acids on biliary epithelial cell proliferation and portal 
      fibroblast activation using rat liver slices.
PG  - 275-85
AB  - During cholestasis, bile acids accumulate in the liver, and induce cellular 
      alterations. Cholestasis is a major cause of liver fibrosis. We have used 
      precision-cut liver slices (PCLS) in culture to investigate the effects of bile 
      acids on hepatic cells. Rat PCLS were placed on an insert in a vial containing 
      culture medium, and gently agitated on a roller platform. PCLS were treated with 
      100 microM taurolithocholate (TLC), taurodeoxycholate (TDC) or taurocholate (TC) 
      for 24 or 48 h. PCLS viability was measured, and immunohistochemistry was 
      performed with antibodies against active caspase 3, platelet-derived growth 
      factor (PDGF) receptor-beta and ED-A fibronectin. TDC and TLC, two hydrophobic 
      bile acids, induced hepatocyte necrosis and apoptosis, whereas TC, an hydrophilic 
      bile acid, improved slice viability as compared with controls. Both TDC and TC 
      induced biliary epithelial cell proliferation, together with portal fibroblast 
      proliferation and activation, as shown by PDGF receptor-beta and ED-A fibronectin 
      expression. TLC induced biliary epithelial cell apoptosis. Our results indicate 
      that individual bile acids induce cell type-specific effects in a complex liver 
      microenvironment. The fact that PCLS support biliary epithelial cell and portal 
      fibroblast proliferation will make this model very useful for the study of the 
      mechanisms involved in portal fibrosis.
FAU - Clouzeau-Girard, Haude
AU  - Clouzeau-Girard H
AD  - INSERM E0362, Universite Victor Segalen Bordeaux 2, Bordeaux, France.
FAU - Guyot, Christelle
AU  - Guyot C
FAU - Combe, Chantal
AU  - Combe C
FAU - Moronvalle-Halley, Valerie
AU  - Moronvalle-Halley V
FAU - Housset, Chantal
AU  - Housset C
FAU - Lamireau, Thierry
AU  - Lamireau T
FAU - Rosenbaum, Jean
AU  - Rosenbaum J
FAU - Desmouliere, Alexis
AU  - Desmouliere A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Cholagogues and Choleretics)
RN  - 0 (Fibronectins)
RN  - 5E090O0G3Z (Taurocholic Acid)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Bile Ducts, Intrahepatic/*drug effects/pathology
MH  - Cell Proliferation/drug effects
MH  - Cholagogues and Choleretics/*toxicity
MH  - Epithelial Cells/*drug effects/pathology
MH  - Fibroblasts/*drug effects/pathology
MH  - Fibronectins/metabolism
MH  - Image Processing, Computer-Assisted
MH  - Liver/*drug effects/metabolism/pathology
MH  - Male
MH  - Necrosis
MH  - Portal System/drug effects/pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Platelet-Derived Growth Factor beta/metabolism
MH  - Taurocholic Acid/*toxicity
EDAT- 2006/01/13 09:00
MHDA- 2006/05/19 09:00
CRDT- 2006/01/13 09:00
PHST- 2006/01/13 09:00 [pubmed]
PHST- 2006/05/19 09:00 [medline]
PHST- 2006/01/13 09:00 [entrez]
AID - S0023-6837(22)60006-0 [pii]
AID - 10.1038/labinvest.3700386 [doi]
PST - ppublish
SO  - Lab Invest. 2006 Mar;86(3):275-85. doi: 10.1038/labinvest.3700386.