PMID- 16403911
OWN - NLM
STAT- MEDLINE
DCOM- 20060524
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 107
IP  - 9
DP  - 2006 May 1
TI  - Allogeneic nonmyeloablative hematopoietic cell transplantation in metastatic 
      colon cancer: tumor-specific T cells directed to a tumor-associated antigen are 
      generated in vivo during GVHD.
PG  - 3795-803
AB  - A pilot study was conducted to evaluate safety and activity of nonmyeloablative 
      allogeneic hematopoietic cell transplantation (HCT) in colorectal carcinoma (CRC) 
      and to determine whether a T-cell response to a tumor-associated antigen (TAA) 
      was induced. Fifteen patients with metastatic CRC underwent HCT from human 
      leukocyte antigen (HLA)-matched siblings after a nonmyeloablative conditioning 
      regimen. All patients engrafted with a median donor T-cell chimerism of 72% at 
      day +56. Eight patients experienced grades II to IV acute graft-versus-host 
      disease (GVHD). Despite progressive disease before HCT, partial remission and 
      disease stabilization longer than 90 days were observed in 1 and 3 patients, 
      respectively. Induction of TAA-specific T cells was evaluated with a 
      carcinoembryonic antigen (CEA)-specific HLA-A(*)0201 pentamer in 6 patients with 
      CRC. CEA-specific CD8(+) T cells were detected in 3 of 3 patients concomitant 
      with GHVD onset, but not in 3 of 3 patients without GVHD. They were also not 
      detected in 9 of 9 control patients with GVHD who received transplants for 
      diagnoses other than CRC. Antitumor activity of CEA-specific T cells was also 
      validated in vitro. In one patient, the induction of CEA-specific T cells was 
      associated with a decrease of serum CEA levels and a partial response. Thus, 
      graft-versus-host reactions associated with allogeneic HCT can trigger the 
      generation of T cells specific for CEA, and this may be associated with a 
      clinical response.
FAU - Carnevale-Schianca, Fabrizio
AU  - Carnevale-Schianca F
AD  - Laboratory of Tumor Immunology, Division of Medical Oncology, Unit of Radiation 
      Therapy, Institute for Cancer Research and Treatment, IRCC, Strada Provinciale 
      No. 140, Candiolo, Turin, Italy.
FAU - Cignetti, Alessandro
AU  - Cignetti A
FAU - Capaldi, Antonio
AU  - Capaldi A
FAU - Vitaggio, Katiuscia
AU  - Vitaggio K
FAU - Vallario, Antonella
AU  - Vallario A
FAU - Ricchiardi, Alberto
AU  - Ricchiardi A
FAU - Sperti, Elisa
AU  - Sperti E
FAU - Ferraris, Renato
AU  - Ferraris R
FAU - Gatti, Marco
AU  - Gatti M
FAU - Grignani, Giovanni
AU  - Grignani G
FAU - Rota-Scalabrini, Delia
AU  - Rota-Scalabrini D
FAU - Geuna, Massimo
AU  - Geuna M
FAU - Fizzotti, Marco
AU  - Fizzotti M
FAU - Sangiolo, Dario
AU  - Sangiolo D
FAU - Sottile, Antonino
AU  - Sottile A
FAU - De Rosa, Giovanni
AU  - De Rosa G
FAU - Bucci, Anna Rosa
AU  - Bucci AR
FAU - Lambertenghi-Deliliers, Giorgio
AU  - Lambertenghi-Deliliers G
FAU - Benedetti, Edoardo
AU  - Benedetti E
FAU - Nash, Richard
AU  - Nash R
FAU - Aglietta, Massimo
AU  - Aglietta M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060110
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*02:01 antigen)
RN  - 0 (HLA-A2 Antigen)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoembryonic Antigen
MH  - Case-Control Studies
MH  - Colonic Neoplasms/immunology/secondary/*therapy
MH  - Female
MH  - Graft vs Host Disease/immunology
MH  - Graft vs Tumor Effect/immunology
MH  - HLA-A Antigens
MH  - HLA-A2 Antigen
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - In Vitro Techniques
MH  - Male
MH  - Middle Aged
MH  - Pilot Projects
MH  - T-Lymphocytes/immunology
MH  - Transplantation Chimera/immunology
MH  - Transplantation, Homologous
EDAT- 2006/01/13 09:00
MHDA- 2006/05/25 09:00
CRDT- 2006/01/13 09:00
PHST- 2006/01/13 09:00 [pubmed]
PHST- 2006/05/25 09:00 [medline]
PHST- 2006/01/13 09:00 [entrez]
AID - S0006-4971(20)65570-7 [pii]
AID - 10.1182/blood-2005-10-3945 [doi]
PST - ppublish
SO  - Blood. 2006 May 1;107(9):3795-803. doi: 10.1182/blood-2005-10-3945. Epub 2006 Jan 
      10.