PMID- 16404508
OWN - NLM
STAT- MEDLINE
DCOM- 20060329
LR  - 20061115
IS  - 1387-2176 (Print)
IS  - 1387-2176 (Linking)
VI  - 7
IP  - 4
DP  - 2005 Dec
TI  - An electrokinetically-controlled immunoassay for simultaneous detection of 
      multiple microbial antigens.
PG  - 301-12
AB  - An electrokinetically-controlled heterogeneous immunoassay microchip for multiple 
      analyte detection was developed in this study. Numerical simulation was employed 
      to study the transport process in a microfluidic network (microFN). The operation 
      parameters obtained from numerical simulation was then applied to immunoassay 
      experiment. The effectiveness of the automatic electrokinetic control was 
      demonstrated in a separate experiment using fluorescein dye. The immunoassay 
      microchip was made of poly(dimethylsiloxane)(PDMS)/PDMS-coated glass using soft 
      lithography and replica molding. Multi-antigen immobilization was accomplished by 
      adsorbing the antigen molecules onto a PDMS-coated glass slide and by using a 
      microFN. Immobilized lysate antigen of Escherichia coli O157: H7 at different 
      concentrations was assayed and the lower detect limit was 3 microg/mL. The assay 
      also displayed very good specificity, when different microbial lysate antigens 
      were immobilized, including Escherichia coli and Helicobacter pylori, and the 
      primary and secondary antibodies were mixtures of different species. The time 
      required for the immunoassay, from antigen coating to signal detection, was only 
      one hour. While still an un-optimized prototype, this automatic-operating, 
      high-throughput immunoassay microchip shows a great potential in detecting 
      multiple pathogenic infections efficiently for clinical applications.
FAU - Gao, Yali
AU  - Gao Y
AD  - Department of Mechanical and Industrial Engineering, University of Toronto, 5 
      King's College Road, Toronto, Ontario M5S 3G8, Canada.
FAU - Hu, Guoqing
AU  - Hu G
FAU - Lin, Frank Y H
AU  - Lin FY
FAU - Sherman, Philip M
AU  - Sherman PM
FAU - Li, Dongqing
AU  - Li D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biomed Microdevices
JT  - Biomedical microdevices
JID - 100887374
RN  - 0 (Antigens, Bacterial)
SB  - IM
MH  - Antigens, Bacterial/*analysis
MH  - Biosensing Techniques/*instrumentation/methods
MH  - *Computer-Aided Design
MH  - Electrochemistry/*instrumentation/methods
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Immunoassay/*instrumentation/methods
MH  - Microarray Analysis/*instrumentation/methods
MH  - Microelectrodes
MH  - Microfluidic Analytical Techniques/*instrumentation/methods
EDAT- 2006/01/13 09:00
MHDA- 2006/03/30 09:00
CRDT- 2006/01/13 09:00
PHST- 2006/01/13 09:00 [pubmed]
PHST- 2006/03/30 09:00 [medline]
PHST- 2006/01/13 09:00 [entrez]
AID - 10.1007/s10544-005-6072-0 [doi]
PST - ppublish
SO  - Biomed Microdevices. 2005 Dec;7(4):301-12. doi: 10.1007/s10544-005-6072-0.