PMID- 16404714
OWN - NLM
STAT- MEDLINE
DCOM- 20060929
LR  - 20191210
IS  - 1549-3296 (Print)
IS  - 1549-3296 (Linking)
VI  - 77
IP  - 2
DP  - 2006 May
TI  - A new biodegradable polyester elastomer for cartilage tissue engineering.
PG  - 331-9
AB  - The objective of this study is to assess whether a new biodegradable elastomer, 
      poly(1,8-octanediol citrate) (POC), would be a suitable material to engineer 
      elastomeric scaffolds for cartilage tissue engineering. Porous POC scaffolds were 
      prepared via the salt-leaching method and initially assessed for their ability to 
      rapidly recover from compressive deformation (% recovery ratio). Controls 
      consisted of scaffolds made from other materials commonly used in cartilage 
      tissue engineering, including 2% agarose, 4% alginate, non woven poly(glycolic 
      acid) (PGA) meshes, and non woven poly(L-lactide-co-glycolide) (PLGA) meshes. 
      Articular chondrocytes from bovine knee were isolated and seeded onto porous 
      disk-shaped POC scaffolds, which were subsequently cultured in vitro for up to 28 
      days. POC scaffolds completely recover from compressive deformation, and the 
      stress-strain curve is typical of an elastomer (recovery ratio>98%). Agarose gel 
      (2%) scaffolds broke during the compression test. The recovery ratio of 4% 
      alginate gel scaffolds, PLLA, and PGA were 72, 85, and 88%, respectively. The 
      Young's modulus of POC-chondrocyte constructs and cell-free POC scaffolds 
      cultured for 28 days were 12.02+/-2.26 kPa and 3.27+/-0.72 kPa, respectively. 
      After 28 days of culture, the recovery ratio of POC-chondrocyte constructs and 
      cell-free POC scaffolds were 93% and 99%, respectively. The glycosaminoglycan 
      (GAG) and collagen content at day 28 was 36% and 26% of that found in bovine knee 
      cartilage explants. Histology/immunohistochemistry evaluations confirm that 
      chondrocytes were able to attach to the pore walls within the scaffold, maintain 
      cell phenotype, and form a cartilaginous tissue during the 28 days of culture.
CI  - Copyright (c) 2006 Wiley Periodicals, Inc.
FAU - Kang, Yong
AU  - Kang Y
AD  - Biomedical Engineering Department, Northwestern University, Evanston, Illinois 
      60208, USA.
FAU - Yang, Jian
AU  - Yang J
FAU - Khan, Sadiya
AU  - Khan S
FAU - Anissian, Lucas
AU  - Anissian L
FAU - Ameer, Guillermo A
AU  - Ameer GA
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Biocompatible Materials)
RN  - 0 (Citrates)
RN  - 0 (Elastomers)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 0 (poly(1,8-octanediol citrate))
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - *Absorbable Implants
MH  - Animals
MH  - Biocompatible Materials/chemistry/*metabolism
MH  - Cartilage, Articular/cytology/pathology/*physiology
MH  - Cattle
MH  - Cells, Cultured
MH  - Chondrocytes/cytology/metabolism
MH  - Citrates/chemistry/*metabolism
MH  - Collagen/metabolism
MH  - Compressive Strength
MH  - Elastomers/chemistry/*metabolism
MH  - Extracellular Matrix/chemistry/metabolism
MH  - Glycosaminoglycans/metabolism
MH  - Humans
MH  - Materials Testing
MH  - Polyesters/chemistry/*metabolism
MH  - Polymers/chemistry/*metabolism
MH  - Stress, Mechanical
MH  - Surface Properties
MH  - *Tissue Engineering/instrumentation/methods
EDAT- 2006/01/13 09:00
MHDA- 2006/09/30 09:00
CRDT- 2006/01/13 09:00
PHST- 2006/01/13 09:00 [pubmed]
PHST- 2006/09/30 09:00 [medline]
PHST- 2006/01/13 09:00 [entrez]
AID - 10.1002/jbm.a.30607 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2006 May;77(2):331-9. doi: 10.1002/jbm.a.30607.