PMID- 16404974
OWN - NLM
STAT- MEDLINE
DCOM- 20060203
LR  - 20191210
IS  - 0869-2084 (Print)
IS  - 0869-2084 (Linking)
IP  - 11
DP  - 2005 Nov
TI  - [Quantitative analysis of fluorescence in situ hybridization (FISH) signals for 
      molecular cytogenetic diagnosis].
PG  - 33-6
AB  - Molecular cytogenetic diagnosis of chromosomal aberrations, by using fluorescence 
      in situ hybridization (FISH) requires the introduction of quantitative approaches 
      to estimating hybridization signals. Specifically, this estimation is required 
      for the differentiation of monosomy and for the superposition of the signals in 
      the diagnosis of mosaic forms of chromosomal syndromes, as well for the 
      determination of the parental origin of homologous chromosomes. The present paper 
      proposes an approach to rapidly estimating FISH signals, based on image capture 
      by a CCD digital chamber and a fluorescence microscope, and to measuring the 
      intensity of hybridization signals. This approach has been shown to be highly 
      effective in differentiating the parent origin of chromosomes, by applying 
      original chromosome-specific DNA tests. It has been successfully used in the 
      study of numerical chromosomal aberrations in various biological materials. By 
      taking into account the findings and as well as earlier described quantitative 
      FISH analyses applied to basic studies, the authors discuss whether the 
      quantitative FISH analyses can be used for the molecular cytogenetic diagnosis of 
      hereditary diseases.
FAU - Iurov, I Iu
AU  - Iurov IIu
FAU - Solov'ev, I V
AU  - Solov'ev IV
FAU - Monakhov, V V
AU  - Monakhov VV
FAU - Vorsanova, S G
AU  - Vorsanova SG
FAU - Iurov, Iu B
AU  - Iurov IuB
LA  - rus
PT  - Evaluation Study
PT  - Journal Article
PL  - Russia (Federation)
TA  - Klin Lab Diagn
JT  - Klinicheskaia laboratornaia diagnostika
JID - 9432021
SB  - IM
MH  - Adult
MH  - Aneuploidy
MH  - Child
MH  - Chromosome Disorders/*diagnosis
MH  - Down Syndrome/diagnosis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
EDAT- 2006/01/13 09:00
MHDA- 2006/02/04 09:00
CRDT- 2006/01/13 09:00
PHST- 2006/01/13 09:00 [pubmed]
PHST- 2006/02/04 09:00 [medline]
PHST- 2006/01/13 09:00 [entrez]
PST - ppublish
SO  - Klin Lab Diagn. 2005 Nov;(11):33-6.