PMID- 16405916
OWN - NLM
STAT- MEDLINE
DCOM- 20060321
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 340
IP  - 4
DP  - 2006 Feb 24
TI  - Suppression of porcine arterivirus replication by baculovirus-delivered shRNA 
      targeting nucleoprotein.
PG  - 1178-83
AB  - The ability to synthesize shRNAs from DNA templates driven by RNA polymerase III 
      promoters has made it possible to apply virus-derived vectors as delivery 
      vehicles for double-strand RNA-mediated interference. Baculovirus emerges as a 
      promising vector for in vivo gene therapy most recently. To investigate its 
      potential as a delivery vector for anti-virus shRNA targeting arterivirus porcine 
      reproductive and respiratory syndrome virus (PRRSV), we constructed recombinant 
      baculovirus vectors bearing a shRNA-synthesizing cassette driven by U6 promoter 
      with enhanced transduction efficiency by displaying vesicular stomatitis virus 
      glycoprotein on viral envelope. Transduction of Marc145 cells with a recombinant 
      baculovirus delivering egfp gene-specific shRNA dramatically suppressed the 
      expression of EGFP in this cell line; and transduction of Marc145 cells with a 
      baculovirus delivering shRNA specific for the C-terminal nucleoprotein coding 
      region of PRRSV genome resulted in inhibition of viral replication. Our data 
      highlight the recombinant baculovirus as an alternative vehicle for anti-virus 
      shRNA delivery.
FAU - Lu, Liqun
AU  - Lu L
AD  - Animal Health Biotechnology Unit, Temasek Life Sciences Laboratory, 1 Research 
      Link, National University of Singapore, Singapore 117604, Singapore.
FAU - Ho, Yuenfern
AU  - Ho Y
FAU - Kwang, Jimmy
AU  - Kwang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060104
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Nucleoproteins)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Baculoviridae/*genetics
MH  - Gene Silencing
MH  - Gene Targeting/*methods
MH  - Nucleoproteins/*genetics
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*growth & 
      development
MH  - RNA, Viral/*genetics
MH  - Suppression, Genetic/genetics
MH  - Transfection/methods
MH  - Virus Replication/*genetics
EDAT- 2006/01/13 09:00
MHDA- 2006/03/22 09:00
CRDT- 2006/01/13 09:00
PHST- 2005/12/15 00:00 [received]
PHST- 2005/12/20 00:00 [accepted]
PHST- 2006/01/13 09:00 [pubmed]
PHST- 2006/03/22 09:00 [medline]
PHST- 2006/01/13 09:00 [entrez]
AID - S0006-291X(05)02889-5 [pii]
AID - 10.1016/j.bbrc.2005.12.133 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Feb 24;340(4):1178-83. doi: 
      10.1016/j.bbrc.2005.12.133. Epub 2006 Jan 4.